primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
rps16-trnQ_p1	rps16-trnQ	1	AAGTCGCACGTTGCTTTCTA	GAGGTTCGAATCCTTCCGTC	20	20	45.0	55.0	58.1	58.1	8.1	16.9	0.215425	5606	7155	1607-1624	1.000000	79.7
rps16-trnQ_p2	rps16-trnQ	2	TCATGTCCTTCAAGTCGCAC	GAGGTTCGAATCCTTCCGTC	20	20	50.0	55.0	58.2	58.1	0.0	16.9	0.280333	5606	7155	1618-1635	1.000000	79.2
rps16-trnQ_p3	rps16-trnQ	3	AAGTCGCACGTTGCTTTCTA	CTCGGAGGTTCGAATCCTTC	20	20	45.0	55.0	58.1	57.8	8.1	16.9	0.350677	5606	7155	1611-1628	1.000000	78.6
rps16-trnQ_p4	rps16-trnQ	4	TCATGTCCTTCAAGTCGCAC	CTCGGAGGTTCGAATCCTTC	20	20	50.0	55.0	58.2	57.8	0.0	16.9	0.415585	5606	7155	1622-1639	1.000000	78.1
rps16-trnQ_p5	rps16-trnQ	5	AAGTCGCACGTTGCTTTCTA	TAAAACCAGAAATGGGGCGT	20	20	45.0	45.0	58.1	57.7	8.1	0.0	0.435397	5606	7155	1667-1684	1.000000	77.9
trnD-psbM_p1	trnD-psbM	1	TTCAATTGGTCAGAGCACCG	TGCGAGAATATTGACTTCCA	20	20	50.0	40.0	58.5	54.5	5.4	0.0	3.996878	17554	18543	1074-1075	1.000000	49.3
trnD-psbM_p2	trnD-psbM	2	TAGGTATGCCATACACCCCG	TGCGAGAATATTGACTTCCA	20	20	55.0	40.0	58.7	54.5	16.3	0.0	4.175481	17554	18543	1106-1107	1.000000	47.8
trnD-psbM_p3	trnD-psbM	3	TTCAATTGGTCAGAGCACCG	ATGCGAGAATATTGACTTCCA	20	21	50.0	38.1	58.5	55.1	5.4	0.0	4.379605	17554	18543	1075-1076	1.000000	46.2
trnD-psbM_p4	trnD-psbM	4	TTCAATTGGTCAGAGCACCG	TGCGAGAATATTGACTTCCAT	20	21	50.0	38.1	58.5	55.1	5.4	0.0	4.379605	17554	18543	1074-1075	1.000000	46.2
trnD-psbM_p5	trnD-psbM	5	GTTCAATTGGTCAGAGCACC	TGCGAGAATATTGACTTCCA	20	20	50.0	40.0	57.0	54.5	5.4	0.0	4.517032	17554	18543	1075-1076	1.000000	45.1
petN-trnC_p1	petN-trnC	1	AGCCCAAGCGAGACTTACTA	AGTTCAAATCTGGGTGCCG	20	19	50.0	52.6	58.2	58.1	0.0	0.0	1.204402	19493	20402	958-959	1.000000	70.6
petN-trnC_p2	petN-trnC	2	AGCCCAAGCGAGACTTACTA	CCAGTTCAAATCTGGGTGCC	20	20	50.0	55.0	58.2	59.1	0.0	4.3	1.260665	19493	20402	960-961	1.000000	70.2
petN-trnC_p3	petN-trnC	3	CCACTCCTCCCCCATACTAC	AGTTCAAATCTGGGTGCCG	20	19	60.0	52.6	58.3	58.1	0.0	0.0	1.334774	19493	20402	1014-1015	1.000000	69.6
petN-trnC_p4	petN-trnC	4	CCACTCCTCCCCCATACTAC	CCAGTTCAAATCTGGGTGCC	20	20	60.0	55.0	58.3	59.1	0.0	4.3	1.391037	19493	20402	1016-1017	1.000000	69.1
petN-trnC_p5	petN-trnC	5	TCCACTCCTCCCCCATACTA	AGTTCAAATCTGGGTGCCG	20	19	55.0	52.6	58.4	58.1	0.0	0.0	1.425206	19493	20402	1015-1016	1.000000	68.8
rpoC2_p1	rpoC2	1	GGGGCATTCTGATTTCGCTA	GAAAACTCTGTTGTTCCGCG	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.051399	27181	27300	406-406	1.000000	82.3
rpoC2_p2	rpoC2	2	CTATTTGCGTACCGACCCAA	GAAAACTCTGTTGTTCCGCG	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.031654	27181	27300	914-914	1.000000	81.1
rpoC2_p3	rpoC2	3	TATTTGCGTACCGACCCAAG	GAAAACTCTGTTGTTCCGCG	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.031654	27181	27300	913-913	1.000000	81.1
rpoC2_p4	rpoC2	4	CTATTTGCGTACCGACCCAA	TGAGATGCATTTCCTCTGGC	20	20	50.0	50.0	58.0	58.0	0.0	14.2	0.061381	27181	27300	1098-1098	1.000000	80.7
rpoC2_p5	rpoC2	5	TATTTGCGTACCGACCCAAG	TGAGATGCATTTCCTCTGGC	20	20	50.0	50.0	58.0	58.0	0.0	14.2	0.061381	27181	27300	1097-1097	1.000000	80.7
atpF_p1	atpF	1	AGAGGATATGCTCGCCTTCT	CATCAACCCATCGCCCATTA	20	20	50.0	50.0	58.0	58.0	17.4	0.0	0.028388	35254	36648	2001-2003	1.000000	80.9
atpF_p2	atpF	2	AGAGGATATGCTCGCCTTCT	TTTTGCCAGTCTGTCTGTCC	20	20	50.0	50.0	58.0	58.0	17.4	0.0	0.040924	35254	36648	2284-2286	1.000000	80.8
atpF_p3	atpF	3	GTATTGCGAGACAGCCAGAA	CATCAACCCATCGCCCATTA	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.030290	35254	36648	2319-2321	1.000000	80.7
atpF_p4	atpF	4	GTATTGCGAGACAGCCAGAA	TTTTGCCAGTCTGTCTGTCC	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.042826	35254	36648	2602-2604	1.000000	80.6
atpF_p5	atpF	5	AGAGGATATGCTCGCCTTCT	CAATTTCGCCTCTCCCATCA	20	20	50.0	50.0	58.0	58.0	17.4	0.0	0.050928	35254	36648	2123-2124	0.666667	66.9
trnR-trnfM_p1	trnR-trnfM	1	AGAAGACCTCTGTCCTATCCA	GTTTGGTAGCTCACAAGGCT	21	20	47.6	50.0	56.9	58.1	0.0	0.0	2.154045	38460	38612	237-239	1.000000	64.5
trnR-trnfM_p2	trnR-trnfM	2	AGAAGACCTCTGTCCTATCCA	TTTGGTAGCTCACAAGGCTC	21	20	47.6	50.0	56.9	57.8	0.0	0.0	2.236609	38460	38612	236-238	1.000000	63.8
trnR-trnfM_p3	trnR-trnfM	3	AGAAGACCTCTGTCCTATCCA	TTATCCACTAGTTCCCCGCT	21	20	47.6	50.0	56.9	57.8	0.0	0.0	2.221617	38460	38612	280-282	1.000000	63.7
trnR-trnfM_p4	trnR-trnfM	4	AGGTTTAGAAGACCTCTGTCCT	GTTTGGTAGCTCACAAGGCT	22	20	45.5	50.0	57.9	58.1	0.0	0.0	2.237373	38460	38612	243-245	1.000000	63.7
trnR-trnfM_p5	trnR-trnfM	5	AGGTTTAGAAGACCTCTGTCCT	TTATCCACTAGTTCCCCGCT	22	20	45.5	50.0	57.9	57.8	0.0	0.0	2.304945	38460	38612	286-288	1.000000	63.0
psaA-ycf3_p1	psaA-ycf3	1	TTTTACTTCTGGTTCCGGCG	TATAGCGCTTACTCCGGGAA	20	20	50.0	50.0	58.5	58.0	0.0	18.7	0.499445	43793	44418	726-735	1.000000	78.2
psaA-ycf3_p2	psaA-ycf3	2	TGTTCTTGAGAAATGGCCGG	TATAGCGCTTACTCCGGGAA	20	20	50.0	50.0	58.5	58.0	0.0	18.7	0.480902	43793	44418	798-807	1.000000	78.1
psaA-ycf3_p3	psaA-ycf3	3	TTTTACTTCTGGTTCCGGCG	GCGCTTACTCCGGGAAATTA	20	20	50.0	50.0	58.5	58.1	0.0	0.0	0.540887	43793	44418	722-731	1.000000	77.9
psaA-ycf3_p4	psaA-ycf3	4	TGTTCTTGAGAAATGGCCGG	GCGCTTACTCCGGGAAATTA	20	20	50.0	50.0	58.5	58.1	0.0	0.0	0.522343	43793	44418	794-803	1.000000	77.8
psaA-ycf3_p5	psaA-ycf3	5	GGTTTGGCCCATTCCTCAAA	TATAGCGCTTACTCCGGGAA	20	20	50.0	50.0	58.7	58.0	0.0	18.7	0.667465	43793	44418	779-788	1.000000	76.7
ycf3-trnS_p1	ycf3-trnS	1	TTTTTCCCCTGAGGTTGTCG	ACGGAAAGAGAGGGATTCGA	20	20	50.0	50.0	58.0	58.2	0.0	0.0	0.175689	46404	46994	778-778	1.000000	80.1
ycf3-trnS_p2	ycf3-trnS	2	TTTTTCCCCTGAGGTTGTCG	TTCCAATGCTACGCCTTGAA	20	20	50.0	45.0	58.0	57.8	0.0	5.6	0.217004	46404	46994	728-728	1.000000	79.9
ycf3-trnS_p3	ycf3-trnS	3	TTTTTCCCCTGAGGTTGTCG	AGTTCCAATGCTACGCCTTG	20	20	50.0	50.0	58.0	58.5	0.0	0.0	0.571186	46404	46994	730-730	1.000000	77.1
ycf3-trnS_p4	ycf3-trnS	4	TTTTTCCCCTGAGGTTGTCG	CAGTTCCAATGCTACGCCTT	20	20	50.0	50.0	58.0	58.5	0.0	0.0	0.571186	46404	46994	731-731	1.000000	77.1
ycf3-trnS_p5	ycf3-trnS	5	TTTTTCCCCTGAGGTTGTCG	TCCAATGCTACGCCTTGAAC	20	20	50.0	50.0	58.0	58.6	0.0	5.6	0.575356	46404	46994	727-727	1.000000	77.0
trnT-trnL_p1	trnT-trnL	1	ATTTGAACCGATGACCCTCG	ACAATCAAGTCCGTAGCGTC	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.032006	48343	49168	919-933	1.000000	81.3
trnT-trnL_p2	trnT-trnL	2	CGATGACCCTCGCATTACAA	ACAATCAAGTCCGTAGCGTC	20	20	50.0	50.0	58.1	58.0	0.0	0.0	0.073242	48343	49168	911-925	1.000000	81.0
trnT-trnL_p3	trnT-trnL	3	GCGATGCTCTAACCTCTGAG	ACAATCAAGTCCGTAGCGTC	20	20	55.0	50.0	57.9	58.0	0.0	0.0	0.140570	48343	49168	889-903	1.000000	80.5
trnT-trnL_p4	trnT-trnL	4	ATTTGAACCGATGACCCTCG	CCGTAGCGTCTACCGATTTC	20	20	50.0	55.0	58.0	58.2	0.0	10.2	0.272624	48343	49168	909-923	1.000000	79.4
trnT-trnL_p5	trnT-trnL	5	ATTTGAACCGATGACCCTCG	CAATCAAGTCCGTAGCGTCT	20	20	50.0	50.0	58.0	57.7	0.0	0.0	0.303758	48343	49168	918-932	1.000000	79.1
trnF-ndhJ_p1	trnF-ndhJ	1	CCTCGTGTCACCAGTTCAAA	TGAAAGTTGGATAGGCTGGC	20	20	50.0	50.0	58.1	57.9	0.0	0.0	0.180666	50214	50796	692-705	1.000000	79.1
trnF-ndhJ_p2	trnF-ndhJ	2	CCTCGTGTCACCAGTTCAAA	ATAGGCTGGCCCTTACGTAA	20	20	50.0	50.0	58.1	58.2	0.0	7.9	0.254575	50214	50796	682-695	1.000000	78.5
trnF-ndhJ_p3	trnF-ndhJ	3	CCTCGTGTCACCAGTTCAAA	ATCATCCACGCCTTAAACGT	20	20	50.0	45.0	58.1	57.6	0.0	0.0	0.448485	50214	50796	723-736	1.000000	77.0
trnF-ndhJ_p4	trnF-ndhJ	4	AATCCTCGTGTCACCAGTTC	TGAAAGTTGGATAGGCTGGC	20	20	50.0	50.0	57.5	57.9	0.0	0.0	0.586136	50214	50796	695-708	1.000000	75.9
trnF-ndhJ_p5	trnF-ndhJ	5	CCTCGTGTCACCAGTTCAAA	GAAAGTTGGATAGGCTGGCC	20	20	50.0	55.0	58.1	58.6	0.0	3.0	0.658293	50214	50796	691-704	1.000000	75.3
ycf4_p1	ycf4	1	CAATTTCAATCGACCGCTGC	TCCTCGTTTGTTCCTTTCTT	20	20	50.0	40.0	58.5	54.8	0.0	0.0	3.643825	60163	60720	694-702	1.000000	51.1
ycf4_p2	ycf4	2	AATTTCAATCGACCGCTGCT	TCCTCGTTTGTTCCTTTCTT	20	20	45.0	40.0	58.5	54.8	0.0	0.0	3.728946	60163	60720	693-701	1.000000	50.4
ycf4_p3	ycf4	3	GACCGCTGCTGGATTTAGTA	TCCTCGTTTGTTCCTTTCTT	20	20	50.0	40.0	57.4	54.8	15.5	0.0	3.779004	60163	60720	683-691	1.000000	50.0
ycf4_p4	ycf4	4	CAATTTCAATCGACCGCTGC	TCCTCGTTTGTTCCTTTCTTT	20	21	50.0	38.1	58.5	55.6	0.0	0.0	3.813966	60163	60720	694-702	1.000000	49.7
ycf4_p5	ycf4	5	CAATTTCAATCGACCGCTGC	ATCCTCGTTTGTTCCTTTCT	20	20	50.0	40.0	58.5	54.6	0.0	0.0	3.883093	60163	60720	695-703	1.000000	49.2
psbE-petL_p1	psbE-petL	1	TGACAAATAACCAACCCGCA	AAGCAGCCAGTAGAAAACCG	20	20	45.0	50.0	58.0	58.5	0.0	0.0	0.493582	64753	65980	1386-1386	1.000000	76.4
psbE-petL_p2	psbE-petL	2	TGACAAATAACCAACCCGCA	GCAGCCAGTAGAAAACCGAA	20	20	45.0	50.0	58.0	58.5	0.0	0.0	0.497931	64753	65980	1384-1384	1.000000	76.4
psbE-petL_p3	psbE-petL	3	CAACCCGCAATGAATAGGGA	AAGCAGCCAGTAGAAAACCG	20	20	50.0	50.0	57.9	58.5	6.4	0.0	0.527577	64753	65980	1375-1375	1.000000	76.1
psbE-petL_p4	psbE-petL	4	CAACCCGCAATGAATAGGGA	GCAGCCAGTAGAAAACCGAA	20	20	50.0	50.0	57.9	58.5	6.4	0.0	0.531926	64753	65980	1373-1373	1.000000	76.1
psbE-petL_p5	psbE-petL	5	ACCAACCCGCAATGAATAGG	AAGCAGCCAGTAGAAAACCG	20	20	50.0	50.0	58.2	58.5	0.0	0.0	0.713535	64753	65980	1377-1377	1.000000	74.6
petB_p1	petB	1	GGTAGTTCGACCGTGGAATT	ACCACAACCTACCCGATATGA	20	21	50.0	47.6	57.6	58.2	0.0	0.0	1.620707	73827	75288	1569-1575	1.000000	67.4
petB_p2	petB	2	GGTAGTTCGACCGTGGAATT	ACCACAACCTACCCGATATG	20	20	50.0	50.0	57.6	56.7	0.0	0.0	1.668360	73827	75288	1569-1575	1.000000	67.1
petB_p3	petB	3	GGTAGTTCGACCGTGGAATT	TGAAATACCACAACCTACCCG	20	21	50.0	47.6	57.6	57.7	0.0	0.0	1.712867	73827	75288	1575-1581	1.000000	66.7
petB_p4	petB	4	ATTCTGGTAGTTCGACCGTG	ACCACAACCTACCCGATATGA	20	21	50.0	47.6	57.4	58.2	0.0	0.0	1.894865	73827	75288	1574-1580	1.000000	65.2
petB_p5	petB	5	CATTCTGGTAGTTCGACCGT	ACCACAACCTACCCGATATGA	20	21	50.0	47.6	57.4	58.2	0.0	0.0	1.894865	73827	75288	1575-1581	1.000000	65.2
petD_p1	petD	1	TGGATACTTTTCTTCAACTCCGA	CACGGTACTTCACGGTTGAA	23	20	39.1	50.0	57.5	58.1	0.0	0.0	3.622093	75477	76708	1350-1355	1.000000	51.1
petD_p2	petD	2	ACTTTTCTTCAACTCCGAAGT	CACGGTACTTCACGGTTGAA	21	20	38.1	50.0	55.4	58.1	0.0	0.0	3.759880	75477	76708	1345-1350	1.000000	50.0
petD_p3	petD	3	TGCTACAAACATGGGTTATTGT	CACGGTACTTCACGGTTGAA	22	20	36.4	50.0	56.2	58.1	0.0	0.0	3.900166	75477	76708	1390-1395	1.000000	48.9
petD_p4	petD	4	TGGATACTTTTCTTCAACTCCGA	CCTATGCACGGTACTTCACG	23	20	39.1	55.0	57.5	58.4	0.0	0.0	3.921043	75477	76708	1356-1361	1.000000	48.7
petD_p5	petD	5	TGGATACTTTTCTTCAACTCCGA	CTATGCACGGTACTTCACGG	23	20	39.1	55.0	57.5	58.4	0.0	0.0	3.921043	75477	76708	1355-1360	1.000000	48.7
petD-rpoA_p1	petD-rpoA	1	ATCGGTACTGCAGTAGCTCT	TGGGACACTCTAGAGAAGCA	20	20	50.0	50.0	57.9	57.4	38.7	26.6	0.664962	76709	76921	354-354	1.000000	76.9
petD-rpoA_p2	petD-rpoA	2	TCGGTACTGCAGTAGCTCTT	TGGGACACTCTAGAGAAGCA	20	20	50.0	50.0	58.2	57.4	38.7	26.6	0.769418	76709	76921	353-353	1.000000	76.1
petD-rpoA_p3	petD-rpoA	3	ATCGGTACTGCAGTAGCTCT	GAGAAGCATCTCCCAATCGA	20	20	50.0	50.0	57.9	57.1	38.7	7.8	0.958779	76709	76921	342-342	1.000000	74.7
petD-rpoA_p4	petD-rpoA	4	CCAGTAGCTACGACCGTTTT	TGGGACACTCTAGAGAAGCA	20	20	50.0	50.0	57.6	57.4	18.0	26.6	0.963851	76709	76921	378-378	1.000000	74.4
petD-rpoA_p5	petD-rpoA	5	TCGGTACTGCAGTAGCTCTT	GAGAAGCATCTCCCAATCGA	20	20	50.0	50.0	58.2	57.1	38.7	7.8	1.063235	76709	76921	341-341	1.000000	73.8
ndhF-rpl32_p1	ndhF-rpl32	1	GGAAGTGGGAGAAGAGGGAT	TCCAAAGATTTTTACGAATACGCT	20	24	55.0	33.3	58.1	57.3	0.0	0.0	4.844538	107323	108145	927-1000	1.000000	43.5
ndhF-rpl32_p2	ndhF-rpl32	2	GGAAGTGGGAGAAGAGGGAT	TGACATCGAAGTACGTTTTT	20	20	55.0	35.0	58.1	53.2	0.0	0.0	4.880333	107323	108145	899-972	1.000000	43.3
ndhF-rpl32_p3	ndhF-rpl32	3	GGAAGTGGGAGAAGAGGGAT	TTGACATCGAAGTACGTTTT	20	20	55.0	35.0	58.1	53.2	0.0	0.0	4.880333	107323	108145	900-973	1.000000	43.3
ndhF-rpl32_p4	ndhF-rpl32	4	GGAAGTGGGAGAAGAGGGAT	TTTGACATCGAAGTACGTTT	20	20	55.0	35.0	58.1	53.2	0.0	0.0	4.880333	107323	108145	901-974	1.000000	43.3
ndhF-rpl32_p5	ndhF-rpl32	5	GGAAGTGGGAGAAGAGGGAT	TTTTGACATCGAAGTACGTT	20	20	55.0	35.0	58.1	53.2	0.0	0.0	4.880333	107323	108145	902-975	1.000000	43.3
rpl32-trnL_p1	rpl32-trnL	1	AAGGGTTTTTCTGGGCAACA	TCCTAAGAGCAGCGTGTCTA	20	20	45.0	50.0	58.1	57.9	0.0	0.0	0.251505	108326	109028	775-794	1.000000	79.2
rpl32-trnL_p2	rpl32-trnL	2	AGGGTTTTTCTGGGCAACAA	TCCTAAGAGCAGCGTGTCTA	20	20	45.0	50.0	58.1	57.9	0.0	0.0	0.251505	108326	109028	774-793	1.000000	79.2
rpl32-trnL_p3	rpl32-trnL	3	AAGGGTTTTTCTGGGCAACA	AGCAGCGTGTCTACCAATTT	20	20	45.0	45.0	58.1	57.8	0.0	0.0	0.312231	108326	109028	768-787	1.000000	78.7
rpl32-trnL_p4	rpl32-trnL	4	AGGGTTTTTCTGGGCAACAA	AGCAGCGTGTCTACCAATTT	20	20	45.0	45.0	58.1	57.8	0.0	0.0	0.312231	108326	109028	767-786	1.000000	78.7
rpl32-trnL_p5	rpl32-trnL	5	AGGGTTTTTCTGGGCAACAA	GCTTGAGCACTGCTTCCTAA	20	20	45.0	50.0	58.1	58.2	0.0	4.7	0.313714	108326	109028	788-807	1.000000	78.7
ndhG-ndhI_p1	ndhG-ndhI	1	CCCAGCGAAAAGGCAGAATA	GAGTCGGTTACCAATCTCCA	20	20	50.0	50.0	58.2	56.7	0.0	1.1	1.591617	113743	113988	483-483	1.000000	68.9
ndhG-ndhI_p2	ndhG-ndhI	2	CCCAGCGAAAAGGCAGAATA	TGAGTCGGTTACCAATCTCC	20	20	50.0	50.0	58.2	56.7	0.0	1.1	1.591617	113743	113988	484-484	1.000000	68.9
ndhG-ndhI_p3	ndhG-ndhI	3	CCCAGCGAAAAGGCAGAATA	TGAGTCGGTTACCAATCTCCA	20	21	50.0	47.6	58.2	58.5	0.0	1.1	1.718036	113743	113988	484-484	1.000000	67.9
ndhG-ndhI_p4	ndhG-ndhI	4	CCCAGCGAAAAGGCAGAATA	TTGAGTCGGTTACCAATCTCC	20	21	50.0	47.6	58.2	57.4	0.0	1.1	1.854682	113743	113988	485-485	1.000000	66.8
ndhG-ndhI_p5	ndhG-ndhI	5	CCCAGCGAAAAGGCAGAATA	GAGTCGGTTACCAATCTCCAT	20	21	50.0	47.6	58.2	57.2	0.0	1.1	2.071228	113743	113988	483-483	1.000000	65.1