Hotspot

rps2

LSC · 15957-16667 · 711 bp

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Pi 0.00281

MarkerSeek score 60.40

Primer available yes

Label rps2

Feature Metadata

label_name: rps2
feature_type: gene
region: LSC
length_bp: 711
variable_sites: 2
pi: 0.00281
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps2_p1	ATATTCTATGGAGTTCCCCC	ACAGAGAAGGTTCCATGGGA	45.0	50.0	53.2	58.0	863-864 bp	1.0	41.4
rps2_p2	TCTATATTCTATGGAGTTCCCCC	ACAGAGAAGGTTCCATGGGA	43.5	50.0	56.1	58.0	866-867 bp	1.0	40.7
rps2_p3	CTATATTCTATGGAGTTCCCCC	ACAGAGAAGGTTCCATGGGA	45.5	50.0	54.6	58.0	865-866 bp	1.0	40.2
rps2_p4	ATCTATATTCTATGGAGTTCCCCC	ACAGAGAAGGTTCCATGGGA	41.7	50.0	56.6	58.0	867-868 bp	1.0	40.2
rps2_p5	ATATTCTATGGAGTTCCCCC	AACAGAGAAGGTTCCATGGG	45.0	50.0	53.2	57.1	864-865 bp	1.0	40.2