Hotspot

rpl14

LSC · 81714-82082 · 369 bp

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Pi 0

MarkerSeek score 28.40

Primer available yes

Label rpl14

Feature Metadata

label_name: rpl14
feature_type: gene
region: LSC
length_bp: 369
variable_sites: 0
pi: 0
unique_haplotype_count: 1
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 28.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl14_p1	AGGTCTCATTCAAATACTATAACT	AGACCTTTGCATTGAAAAAGA	29.2	33.3	53.0	54.2	453 bp	1.0	40.0
rpl14_p2	AGGTCTCATTCAAATACTATAACT	AGACCTTTGCATTGAAAAAGAA	29.2	31.8	53.0	55.0	453 bp	1.0	40.0
rpl14_p3	AGGTCTCATTCAAATACTATAACT	AGACCTTTGCATTGAAAAAGAAA	29.2	30.4	53.0	55.8	453 bp	1.0	40.0
rpl14_p4	AGGTCTCATTCAAATACTATAACT	GACCTTTGCATTGAAAAAGA	29.2	35.0	53.0	52.5	452 bp	1.0	40.0
rpl14_p5	ACTATAACTATATTACGGTCTTACC	AGACCTTTGCATTGAAAAAGA	32.0	33.3	53.3	54.2	438 bp	1.0	40.0