Hotspot

psbA

LSC · 337-1398 · 1062 bp

Back to Aruncus

Pi 0.000942

MarkerSeek score 55.60

Primer available yes

Label psbA

Feature Metadata

label_name: psbA
feature_type: gene
region: LSC
length_bp: 1062
variable_sites: 1
pi: 0.000942
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 55.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbA_p1	GCTCCTTTACTTTCAAGAACTCG	GTGCTTGGGAGTACCTGATG	43.5	55.0	57.7	58.0	1155 bp	1.0	53.7
psbA_p2	GCTCCTTTACTTTCAAGAACTCG	TGCTTGGGAGTACCTGATGA	43.5	50.0	57.7	58.0	1154 bp	1.0	53.5
psbA_p3	GCTCCTTTACTTTCAAGAACTCG	ATTCGTGTGCTTGGGAGTAC	43.5	50.0	57.7	57.9	1161 bp	1.0	53.1
psbA_p4	GCTCCTTTACTTTCAAGAACTCG	AGAATTCGTGTGCTTGGGAG	43.5	50.0	57.7	58.2	1164 bp	1.0	52.4
psbA_p5	GCTCCTTTACTTTCAAGAACTCG	AGAGAATTCGTGTGCTTGGG	43.5	50.0	57.7	58.2	1166 bp	1.0	52.4