Hotspot

ndhF

SSC · 111564-113828 · 2265 bp

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Pi 0.00265

MarkerSeek score 58.70

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: SSC
length_bp: 2265
variable_sites: 6
pi: 0.00265
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 58.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	TCCGAGTGAATGGAAAGGAA	ACGAACCGGTGAATCAGAAA	45.0	45.0	56.5	57.5	2425 bp	1.0	63.6
ndhF_p2	GATCCGAGTGAATGGAAAGGA	ACGAACCGGTGAATCAGAAA	47.6	45.0	57.5	57.5	2427 bp	1.0	63.6
ndhF_p3	AGATCCGAGTGAATGGAAAGG	ACGAACCGGTGAATCAGAAA	47.6	45.0	57.4	57.5	2428 bp	1.0	63.5
ndhF_p4	AACGAAAGAGATCCGAGTGA	ACGAACCGGTGAATCAGAAA	45.0	45.0	56.3	57.5	2436 bp	1.0	62.4
ndhF_p5	ACGAAAGAGATCCGAGTGAA	ACGAACCGGTGAATCAGAAA	45.0	45.0	56.3	57.5	2435 bp	1.0	62.4