Hotspot

ndhC

LSC · 51628-51990 · 363 bp

Back to Aruncus

Pi 0

MarkerSeek score 26.40

Primer available yes

Label ndhC

Feature Metadata

label_name: ndhC
feature_type: gene
region: LSC
length_bp: 363
variable_sites: 0
pi: 0
unique_haplotype_count: 1
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 26.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC_p1	TTTGGGTTGTTCGATCAAGT	TTCGAATCGTCTAATCCGGT	40.0	45.0	55.5	56.5	510 bp	1.0	47.7
ndhC_p2	GGGTTGTTCGATCAAGTAAAGG	TTCGAATCGTCTAATCCGGT	45.5	45.0	57.3	56.5	507 bp	1.0	46.3
ndhC_p3	ACTGAATTTTGGGTTGTTCGA	TTCGAATCGTCTAATCCGGT	38.1	45.0	56.3	56.5	517 bp	1.0	46.0
ndhC_p4	TTTTGGGTTGTTCGATCAAGT	TTCGAATCGTCTAATCCGGT	38.1	45.0	56.3	56.5	511 bp	1.0	46.0
ndhC_p5	TTTGGGTTGTTCGATCAAGT	ATTCGAATCGTCTAATCCGGT	40.0	42.9	55.5	57.0	511 bp	1.0	43.9