Hotspot

clpP

LSC · 71027-72916 · 1890 bp

Back to Aruncus

Pi 0.00372

MarkerSeek score 47.40

Primer available yes

Label clpP

Feature Metadata

label_name: clpP
feature_type: gene
region: LSC
length_bp: 1890
variable_sites: 7
pi: 0.00372
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 47.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
clpP_p1	TGACCCTGTTAGTCCGTTCT	CTGCTGTGGACCCTCTTTTT	50.0	50.0	58.0	58.0	2878-2887 bp	1.0	80.4
clpP_p2	TTCGCTGGTACTGATCGTTG	CTGCTGTGGACCCTCTTTTT	50.0	50.0	58.0	58.0	2643-2652 bp	1.0	80.4
clpP_p3	TTCGCTGGTACTGATCGTTG	TTCGTTACAGTACCCCCTGT	50.0	50.0	58.0	58.0	2965-2974 bp	1.0	80.4
clpP_p4	TTCGCTGGTACTGATCGTTG	TCAAACGACCGGGATCATTC	50.0	50.0	58.0	58.0	2763-2772 bp	1.0	80.4
clpP_p5	CTGAACGAGTCGCACATACA	CTGCTGTGGACCCTCTTTTT	50.0	50.0	58.0	58.0	2580-2589 bp	1.0	80.3