Hotspot

ndhF-rpl32

SSC · 113829-114957 · 1129 bp

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Pi 0.00363

MarkerSeek score 63.10

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 1129
variable_sites: 4
pi: 0.00363
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 63.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	TTGTTGCCGTTGGAAAAAGT	CGCCCAATATCCCTTCCTTT	40.0	50.0	57.0	57.6	1285-1310 bp	1.0	70.6
ndhF-rpl32_p2	TTGTTGCCGTTGGAAAAAGT	AAAAAGCTTTCAATGCCGCC	40.0	45.0	57.0	58.5	1301-1326 bp	1.0	69.6
ndhF-rpl32_p3	TTTGTTGCCGTTGGAAAAAGT	CGCCCAATATCCCTTCCTTT	38.1	50.0	57.7	57.6	1286-1311 bp	1.0	68.4
ndhF-rpl32_p4	TTGTTGCCGTTGGAAAAAGT	TTCAATGCCGCCCAATATCC	40.0	50.0	57.0	58.7	1293-1318 bp	1.0	68.1
ndhF-rpl32_p5	TTTGTTGCCGTTGGAAAAAGT	AAAAAGCTTTCAATGCCGCC	38.1	45.0	57.7	58.5	1302-1327 bp	1.0	67.4