Hotspot

psbE-petL

LSC · 65568-66887 · 1320 bp

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Pi 0.00455

MarkerSeek score 61.40

Primer available yes

Label psbE-petL

Feature Metadata

label_name: psbE-petL
feature_type: igs
region: LSC
length_bp: 1320
variable_sites: 6
pi: 0.00455
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE-petL_p1	TGACAAATAACCAACCCGCA	GTCGTATCTTGCTCAGACCG	45.0	55.0	58.0	58.2	1522-1523 bp	1.0	78.9
psbE-petL_p2	TGACAAATAACCAACCCGCA	TCGTATCTTGCTCAGACCGA	45.0	50.0	58.0	58.3	1521-1522 bp	1.0	78.2
psbE-petL_p3	AGAACGTTCTCCTGTGCTTC	GTCGTATCTTGCTCAGACCG	50.0	55.0	57.8	58.2	1434-1435 bp	1.0	77.8
psbE-petL_p4	GTTCTCCTGTGCTTCCAGAC	GTCGTATCTTGCTCAGACCG	55.0	55.0	58.2	58.2	1429-1430 bp	1.0	77.4
psbE-petL_p5	AGAACGTTCTCCTGTGCTTC	TCGTATCTTGCTCAGACCGA	50.0	50.0	57.8	58.3	1433-1434 bp	1.0	77.1