Hotspot

accD-psaI

LSC · 59806-60576 · 771 bp

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Pi 0.00131

MarkerSeek score 60.50

Primer available yes

Label accD-psaI

Feature Metadata

label_name: accD-psaI
feature_type: igs
region: LSC
length_bp: 771
variable_sites: 1
pi: 0.00131
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 60.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD-psaI_p1	AAAGGGCGTTCTGAATGAGT	CCATTGCAATTGCCGGAAAT	45.0	45.0	57.4	58.0	897-905 bp	1.0	76.0
accD-psaI_p2	AAGGGCGTTCTGAATGAGTT	CCATTGCAATTGCCGGAAAT	45.0	45.0	57.4	58.0	896-904 bp	1.0	76.0
accD-psaI_p3	AAAGGGCGTTCTGAATGAGT	TTGCCGGAAATACTAAGCCC	45.0	50.0	57.4	58.0	888-896 bp	1.0	75.9
accD-psaI_p4	AAGGGCGTTCTGAATGAGTT	TTGCCGGAAATACTAAGCCC	45.0	50.0	57.4	58.0	887-895 bp	1.0	75.9
accD-psaI_p5	AAGGGCGTTCTGAATGAGTT	AAATGGAGGGGAAGCTGTTG	45.0	50.0	57.4	58.1	854-862 bp	1.0	75.7