Hotspot

ndhC-trnV-UAC

LSC · 51991-52426 · 436 bp

Back to Aruncus

Pi 0.0138

MarkerSeek score 63.20

Primer available yes

Label ndhC-trnV-UAC

Feature Metadata

label_name: ndhC-trnV-UAC
feature_type: igs
region: LSC
length_bp: 436
variable_sites: 6
pi: 0.0138
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-trnV-UAC_p1	GGGGCTAAAACTCCGGAAAT	GCACGTTGGGTCTTTGAAAC	50.0	50.0	57.9	58.2	631 bp	1.0	78.3
ndhC-trnV-UAC_p2	GGGGCTAAAACTCCGGAAAT	AGAAGGTCTACGGTTCGAGT	50.0	50.0	57.9	57.8	567 bp	1.0	78.2
ndhC-trnV-UAC_p3	TTCTGGCCCTTTGCTAATCG	GCACGTTGGGTCTTTGAAAC	50.0	50.0	58.2	58.2	650 bp	1.0	77.4
ndhC-trnV-UAC_p4	TTCTGGCCCTTTGCTAATCG	AGAAGGTCTACGGTTCGAGT	50.0	50.0	58.2	57.8	586 bp	1.0	77.2
ndhC-trnV-UAC_p5	GGGGCTAAAACTCCGGAAAT	ATGCACGTTGGGTCTTTGAA	50.0	45.0	57.9	58.3	633 bp	1.0	77.1