Hotspot

atpF-atpH

LSC · 13368-13852 · 485 bp

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Pi 0.00625

MarkerSeek score 60.90

Primer available yes

Label atpF-atpH

Feature Metadata

label_name: atpF-atpH
feature_type: igs
region: LSC
length_bp: 485
variable_sites: 3
pi: 0.00625
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 60.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpF-atpH_p1	ATTAAACCCGAAACTCCCGG	CGGAGGGAAAAATACGAGGT	50.0	50.0	57.9	57.3	662-667 bp	1.0	74.5
atpF-atpH_p2	ATTAAACCCGAAACTCCCGG	GAGGCGGAGGGAAAAATACG	50.0	55.0	57.9	58.7	666-671 bp	1.0	74.4
atpF-atpH_p3	ATTAAACCCGAAACTCCCGG	GCGGAGGGAAAAATACGAGG	50.0	55.0	57.9	58.7	663-668 bp	1.0	74.4
atpF-atpH_p4	ATTAAACCCGAAACTCCCGG	GGCGGAGGGAAAAATACGAG	50.0	55.0	57.9	58.7	664-669 bp	1.0	74.4
atpF-atpH_p5	ATTAAACCCGAAACTCCCGG	CAGAGGCGGAGGGAAAAATA	50.0	50.0	57.9	57.3	668-673 bp	1.0	74.3