Hotspot

atpA-atpF

LSC · 12032-12083 · 52 bp

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Pi 0.0192

MarkerSeek score 63.60

Primer available yes

Label atpA-atpF

Feature Metadata

label_name: atpA-atpF
feature_type: igs
region: LSC
length_bp: 52
variable_sites: 1
pi: 0.0192
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpA-atpF_p1	CCGTCGCCTACTTGAAGTAC	ACAAGGAGCTCTAGGAACTCT	55.0	47.6	58.1	57.5	274-275 bp	1.0	67.8
atpA-atpF_p2	CCGTCGCCTACTTGAAGTAC	AGGAGCTCTAGGAACTCTGA	55.0	50.0	58.1	56.5	271-272 bp	1.0	67.4
atpA-atpF_p3	GCTCAATACGTTCGCGGATA	ACAAGGAGCTCTAGGAACTCT	50.0	47.6	58.2	57.5	216-217 bp	1.0	66.7
atpA-atpF_p4	GCTCAATACGTTCGCGGATA	AGGAGCTCTAGGAACTCTGA	50.0	50.0	58.2	56.5	213-214 bp	1.0	66.4
atpA-atpF_p5	CCGTCGCCTACTTGAAGTAC	AGGAGCTCTAGGAACTCTGAA	55.0	47.6	58.1	57.3	271-272 bp	1.0	65.5