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trnK-UUU-rps16

LSC · 4252-5178 · 927 bp

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Pi 0.00755

MarkerSeek score 63.10

Primer available yes

Label trnK-UUU-rps16

Feature Metadata

label_name: trnK-UUU-rps16
feature_type: igs
region: LSC
length_bp: 927
variable_sites: 7
pi: 0.00755
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU-rps16_p1	TGGTCTTACAAACTCTGCCG	AAAAAGGCGCTCAACCTACA	50.0	45.0	57.8	58.0	1152 bp	1.0	79.0
trnK-UUU-rps16_p2	AAAGCCGAGTACTCTACCGT	AAAAAGGCGCTCAACCTACA	50.0	45.0	58.2	58.0	1074 bp	1.0	79.0
trnK-UUU-rps16_p3	AAGCCGAGTACTCTACCGTT	AAAAAGGCGCTCAACCTACA	50.0	45.0	58.2	58.0	1073 bp	1.0	79.0
trnK-UUU-rps16_p4	CCGCACTTAAAAGCCGAGTA	AAAAAGGCGCTCAACCTACA	50.0	45.0	58.3	58.0	1083 bp	1.0	78.1
trnK-UUU-rps16_p5	AAACTCTGCCGATGGTATGG	AAAAAGGCGCTCAACCTACA	50.0	45.0	57.7	58.0	1143 bp	1.0	77.6