Hotspot

rpoC2

LSC · 17971-22152 · 4182 bp

Back to Artocarpus

Pi 0.00827

MarkerSeek score 39.10

Primer available yes

Label rpoC2

Feature Metadata

label_name: rpoC2
feature_type: gene
region: LSC
length_bp: 4182
variable_sites: 76
pi: 0.00827
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 39.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoC2_p1	TTCTCCCGTTTTCGTCCATT	TGGGTCCATTCAACGTCAAC	45.0	50.0	57.4	58.4	243 bp	1.0	79.3
rpoC2_p2	AATTCTCCCGTTTTCGTCCA	TGGGTCCATTCAACGTCAAC	45.0	50.0	57.4	58.4	245 bp	1.0	79.2
rpoC2_p3	TTCTCCCGTTTTCGTCCATT	GGTCCATTCAACGTCAACGA	45.0	50.0	57.4	58.5	241 bp	1.0	78.6
rpoC2_p4	AATTCTCCCGTTTTCGTCCA	GGTCCATTCAACGTCAACGA	45.0	50.0	57.4	58.5	243 bp	1.0	78.5
rpoC2_p5	TTCTCCCGTTTTCGTCCATT	ATGGGTCCATTCAACGTCAA	45.0	45.0	57.4	57.4	244 bp	1.0	77.8