Hotspot

rpl22

LSC · 88020-88397 · 378 bp

Back to Artocarpus

Pi 0.0162

MarkerSeek score 39.10

Primer available yes

Label rpl22

Feature Metadata

label_name: rpl22
feature_type: gene
region: LSC
length_bp: 378
variable_sites: 13
pi: 0.0162
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 39.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl22_p1	TGCACCAAGTCTGAAACCAA	TCCCGAGCGTCTACCATTAT	45.0	50.0	57.9	58.0	835-853 bp	1.0	85.1
rpl22_p2	AGCCCTAATGGTTTGTAGCG	TCCCGAGCGTCTACCATTAT	50.0	50.0	58.0	58.0	1360-1378 bp	1.0	84.2
rpl22_p3	AGCCCTAATGGTTTGTAGCG	GGGCCCCAATTGGTAGAAAA	50.0	50.0	58.0	58.1	1651-1670 bp	1.0	83.5
rpl22_p4	AGCCCTAATGGTTTGTAGCG	TTGATTCTTCGTCGCCGTAG	50.0	50.0	58.0	58.1	1559-1578 bp	1.0	83.3
rpl22_p5	AGCCCTAATGGTTTGTAGCG	AAAAGTTTGGGTAGAGCCGG	50.0	50.0	58.0	58.1	1757-1776 bp	1.0	82.9