Hotspot

ccsA-ndhD

SSC · 121112-121404 · 293 bp

Back to Artocarpus

Pi 0.0291

MarkerSeek score 54.20

Primer available yes

Label ccsA-ndhD

Feature Metadata

label_name: ccsA-ndhD
feature_type: igs
region: SSC
length_bp: 293
variable_sites: 17
pi: 0.0291
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 54.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA-ndhD_p1	CCCTGGGCGAGATTCAATAC	TACAGGACTCAATGGAGCCA	55.0	50.0	58.1	58.0	1230-1239 bp	1.0	81.6
ccsA-ndhD_p2	GGGAGCAGTATGGGCTAATG	TACAGGACTCAATGGAGCCA	55.0	50.0	57.8	58.0	1064-1073 bp	1.0	81.2
ccsA-ndhD_p3	TCCCTGGGCGAGATTCAATA	TACAGGACTCAATGGAGCCA	50.0	50.0	58.2	58.0	1231-1240 bp	1.0	80.9
ccsA-ndhD_p4	AATGAGTCGTTTTCCCTGGG	TACAGGACTCAATGGAGCCA	50.0	50.0	57.8	58.0	1243-1252 bp	1.0	80.9
ccsA-ndhD_p5	TACCAGCTCTCCAATCCCAT	TACAGGACTCAATGGAGCCA	50.0	50.0	57.8	58.0	1398-1407 bp	0.875	75.5