Hotspot

rpl32-trnL-UAG

SSC · 118243-119958 · 1716 bp

Back to Artocarpus

Pi 0.026

MarkerSeek score 67.60

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: SSC
length_bp: 1716
variable_sites: 102
pi: 0.026
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 67.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	GGGCAGCATTGAAAGCTTTT	AGCGTGTCTACCGATTTCAC	45.0	50.0	57.8	58.0	1814-1907 bp	1.0	87.0
rpl32-trnL-UAG_p2	GGGCAGCATTGAAAGCTTTT	CAGCGTGTCTACCGATTTCA	45.0	50.0	57.8	58.0	1815-1908 bp	1.0	87.0
rpl32-trnL-UAG_p3	GGGCAGCATTGAAAGCTTTT	TAGGAGATATCATGCCGCCA	45.0	50.0	57.8	58.1	1877-1970 bp	1.0	86.4
rpl32-trnL-UAG_p4	GGGCAGCATTGAAAGCTTTT	TCCTAAGAGCAGCGTGTCTA	45.0	50.0	57.8	57.9	1824-1917 bp	1.0	86.0
rpl32-trnL-UAG_p5	GGGCAGCATTGAAAGCTTTT	CTAAGAGCAGCGTGTCTACC	45.0	55.0	57.8	57.8	1822-1915 bp	1.0	85.4