Hotspot

ndhF-rpl32

SSC · 116761-118083 · 1323 bp

Back to Artocarpus

Pi 0.0208

MarkerSeek score 56.80

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 1323
variable_sites: 60
pi: 0.0208
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 56.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	GCTGCAGTAGATCGTGTGAA	AAAAGCTTTCAATGCTGCCC	50.0	45.0	58.0	57.8	1924-2026 bp	1.0	82.4
ndhF-rpl32_p2	GCTGCAGTAGATCGTGTGAA	CTACGTTTCGAGCCAGAACA	50.0	50.0	58.0	57.9	2030-2076 bp	0.625	68.0
ndhF-rpl32_p3	GCTGCAGTAGATCGTGTGAA	ACTACGTTTCGAGCCAGAAC	50.0	50.0	58.0	57.9	2031-2077 bp	0.625	68.0
ndhF-rpl32_p4	AATCTCCGACGCGATTAGTG	CTACGTTTCGAGCCAGAACA	50.0	50.0	57.9	57.9	2074-2120 bp	0.625	67.1
ndhF-rpl32_p5	AATCTCCGACGCGATTAGTG	ACTACGTTTCGAGCCAGAAC	50.0	50.0	57.9	57.9	2075-2121 bp	0.625	67.1