Hotspot

rpl22-rps19

LSC · 88398-88512 · 115 bp

Back to Artocarpus

Pi 0.0416

MarkerSeek score 60.50

Primer available yes

Label rpl22-rps19

Feature Metadata

label_name: rpl22-rps19
feature_type: igs
region: LSC
length_bp: 115
variable_sites: 9
pi: 0.0416
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 60.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl22-rps19_p1	TGCACCAAGTCTGAAACCAA	TCCCGAGCGTCTACCATTAT	45.0	50.0	57.9	58.0	835-853 bp	1.0	85.1
rpl22-rps19_p2	TTGCACCAAGTCTGAAACCA	TCCCGAGCGTCTACCATTAT	45.0	50.0	57.9	58.0	836-854 bp	1.0	85.1
rpl22-rps19_p3	CCGTTGACTTCCGCTTTACT	TCCCGAGCGTCTACCATTAT	50.0	50.0	58.2	58.0	536-555 bp	1.0	84.2
rpl22-rps19_p4	TGCACCAAGTCTGAAACCAA	GGGCCCCAATTGGTAGAAAA	45.0	50.0	57.9	58.1	1126-1145 bp	1.0	83.8
rpl22-rps19_p5	TTGCACCAAGTCTGAAACCA	GGGCCCCAATTGGTAGAAAA	45.0	50.0	57.9	58.1	1127-1146 bp	1.0	83.8