Pi
0.0174
MarkerSeek score
57.40
Primer available
yes
Label
trnT-UGU-trnL-UAA
Feature Metadata
Pi Curve
Haplotype Network
Species Separation
Primer Ranking
| Primer ID | fwd_seq | rev_seq | fwd_gc | rev_gc | fwd_tm | rev_tm | amplicon_size | cross_species_success_rate | primer_score |
|---|---|---|---|---|---|---|---|---|---|
| trnT-UGU-trnL-UAA_p1 | CGATGACCCTCGCATTACAA | ACCAATTTCGCCATATCCCC | 50.0 | 50.0 | 58.1 | 58.0 | 1280-1399 bp | 1.0 | 86.6 |
| trnT-UGU-trnL-UAA_p2 | CGATGACCCTCGCATTACAA | TAGCGTCTACCAATTTCGCC | 50.0 | 50.0 | 58.1 | 58.1 | 1288-1407 bp | 1.0 | 86.1 |
| trnT-UGU-trnL-UAA_p3 | GCGATGCTCTAACCTCTGAG | ACCAATTTCGCCATATCCCC | 55.0 | 50.0 | 57.9 | 58.0 | 1258-1377 bp | 1.0 | 86.1 |
| trnT-UGU-trnL-UAA_p4 | GCGATGCTCTAACCTCTGAG | TAGCGTCTACCAATTTCGCC | 55.0 | 50.0 | 57.9 | 58.1 | 1266-1385 bp | 1.0 | 85.6 |
| trnT-UGU-trnL-UAA_p5 | AAAGCCAACTATCGGAGTCG | ACCAATTTCGCCATATCCCC | 50.0 | 50.0 | 57.7 | 58.0 | 1303-1422 bp | 1.0 | 84.7 |