Hotspot

trnK-UUU-rps16

LSC · 4541-5587 · 1047 bp

Back to Artocarpus

Pi 0.0121

MarkerSeek score 54.30

Primer available yes

Label trnK-UUU-rps16

Feature Metadata

label_name: trnK-UUU-rps16
feature_type: igs
region: LSC
length_bp: 1047
variable_sites: 29
pi: 0.0121
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 54.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU-rps16_p1	AAAGCCGAGTACTCTACCGT	TATTTCAAAGAAGGCGGGGG	50.0	50.0	58.2	57.9	1140-1174 bp	1.0	81.1
trnK-UUU-rps16_p2	AAGCCGAGTACTCTACCGTT	TATTTCAAAGAAGGCGGGGG	50.0	50.0	58.2	57.9	1139-1173 bp	1.0	81.1
trnK-UUU-rps16_p3	CCGCACTTAAAAGCCGAGTA	TATTTCAAAGAAGGCGGGGG	50.0	50.0	58.3	57.9	1149-1183 bp	1.0	80.1
trnK-UUU-rps16_p4	AAAGCCGAGTACTCTACCGT	AGAAGGCGGGGGTTTTTATG	50.0	50.0	58.2	58.2	1132-1162 bp	0.5	59.1
trnK-UUU-rps16_p5	AAGCCGAGTACTCTACCGTT	AGAAGGCGGGGGTTTTTATG	50.0	50.0	58.2	58.2	1131-1161 bp	0.5	59.1