Hotspot

matK-trnK-UUU

LSC · 3704-4503 · 800 bp

Back to Artocarpus

Pi 0.014

MarkerSeek score 56.30

Primer available yes

Label matK-trnK-UUU

Feature Metadata

label_name: matK-trnK-UUU
feature_type: igs
region: LSC
length_bp: 800
variable_sites: 27
pi: 0.014
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 56.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
matK-trnK-UUU_p1	GACTCCCGAAAGATAAGTGGG	AACGGTAGAGTACTCGGCTT	52.4	50.0	57.8	58.2	878-904 bp	1.0	74.2
matK-trnK-UUU_p2	GACTCCCGAAAGATAAGTGGG	ACGGTAGAGTACTCGGCTTT	52.4	50.0	57.8	58.2	877-903 bp	1.0	74.2
matK-trnK-UUU_p3	ACTCCCGAAAGATAAGTGGG	ACGGTAGAGTACTCGGCTTT	50.0	50.0	56.6	58.2	876-902 bp	1.0	72.6
matK-trnK-UUU_p4	CTCCCGAAAGATAAGTGGGT	AACGGTAGAGTACTCGGCTT	50.0	50.0	56.6	58.2	876-902 bp	1.0	72.6
matK-trnK-UUU_p5	CTCCCGAAAGATAAGTGGGT	ACGGTAGAGTACTCGGCTTT	50.0	50.0	56.6	58.2	875-901 bp	1.0	72.6