Hotspot

trnH-GUG-psbA

LSC · 109-536 · 428 bp

Back to Artocarpus

Pi 0.0237

MarkerSeek score 60.30

Primer available yes

Label trnH-GUG-psbA

Feature Metadata

label_name: trnH-GUG-psbA
feature_type: igs
region: LSC
length_bp: 428
variable_sites: 25
pi: 0.0237
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.93
markerseek_score: 60.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	GATCCACTTGGCTACATCCG	GGAAGTTATGCACGAGCGTA	55.0	50.0	58.1	58.1	498-550 bp	1.0	86.1
trnH-GUG-psbA_p2	CAATCCACTGCCTTGATCCA	GGAAGTTATGCACGAGCGTA	50.0	50.0	57.9	58.1	512-564 bp	1.0	85.9
trnH-GUG-psbA_p3	AATCCACTGCCTTGATCCAC	GGAAGTTATGCACGAGCGTA	50.0	50.0	57.9	58.1	511-563 bp	1.0	85.9
trnH-GUG-psbA_p4	ACAATCCACTGCCTTGATCC	GGAAGTTATGCACGAGCGTA	50.0	50.0	57.9	58.1	513-565 bp	1.0	85.9
trnH-GUG-psbA_p5	CGCATGGTGGATTCACAATC	GGAAGTTATGCACGAGCGTA	50.0	50.0	57.5	58.1	527-579 bp	1.0	83.1