primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	trnH-GUG-psbA	1	GATCCACTTGGCTACATCCG	GGAAGTTATGCACGAGCGTA	20	20	55.0	50.0	58.1	58.1	0.0	2.4	0.211647	109	536	498-550	1.000000	86.1
trnH-GUG-psbA_p2	trnH-GUG-psbA	2	CAATCCACTGCCTTGATCCA	GGAAGTTATGCACGAGCGTA	20	20	50.0	50.0	57.9	58.1	0.0	2.4	0.216009	109	536	512-564	1.000000	85.9
trnH-GUG-psbA_p3	trnH-GUG-psbA	3	AATCCACTGCCTTGATCCAC	GGAAGTTATGCACGAGCGTA	20	20	50.0	50.0	57.9	58.1	0.0	2.4	0.217516	109	536	511-563	1.000000	85.9
trnH-GUG-psbA_p4	trnH-GUG-psbA	4	ACAATCCACTGCCTTGATCC	GGAAGTTATGCACGAGCGTA	20	20	50.0	50.0	57.9	58.1	0.0	2.4	0.217516	109	536	513-565	1.000000	85.9
trnH-GUG-psbA_p5	trnH-GUG-psbA	5	CGCATGGTGGATTCACAATC	GGAAGTTATGCACGAGCGTA	20	20	50.0	50.0	57.5	58.1	6.4	2.4	0.562951	109	536	527-579	1.000000	83.1
matK-trnK-UUU_p1	matK-trnK-UUU	1	GACTCCCGAAAGATAAGTGGG	AACGGTAGAGTACTCGGCTT	21	20	52.4	50.0	57.8	58.2	0.0	17.1	1.349311	3704	4503	878-904	1.000000	74.2
matK-trnK-UUU_p2	matK-trnK-UUU	2	GACTCCCGAAAGATAAGTGGG	ACGGTAGAGTACTCGGCTTT	21	20	52.4	50.0	57.8	58.2	0.0	17.1	1.349311	3704	4503	877-903	1.000000	74.2
matK-trnK-UUU_p3	matK-trnK-UUU	3	ACTCCCGAAAGATAAGTGGG	ACGGTAGAGTACTCGGCTTT	20	20	50.0	50.0	56.6	58.2	0.0	17.1	1.542436	3704	4503	876-902	1.000000	72.6
matK-trnK-UUU_p4	matK-trnK-UUU	4	CTCCCGAAAGATAAGTGGGT	AACGGTAGAGTACTCGGCTT	20	20	50.0	50.0	56.6	58.2	0.0	17.1	1.542436	3704	4503	876-902	1.000000	72.6
matK-trnK-UUU_p5	matK-trnK-UUU	5	CTCCCGAAAGATAAGTGGGT	ACGGTAGAGTACTCGGCTTT	20	20	50.0	50.0	56.6	58.2	0.0	17.1	1.542436	3704	4503	875-901	1.000000	72.6
trnK-UUU-rps16_p1	trnK-UUU-rps16	1	AAAGCCGAGTACTCTACCGT	TATTTCAAAGAAGGCGGGGG	20	20	50.0	50.0	58.2	57.9	20.3	0.0	0.306230	4541	5587	1140-1174	1.000000	81.1
trnK-UUU-rps16_p2	trnK-UUU-rps16	2	AAGCCGAGTACTCTACCGTT	TATTTCAAAGAAGGCGGGGG	20	20	50.0	50.0	58.2	57.9	20.3	0.0	0.306230	4541	5587	1139-1173	1.000000	81.1
trnK-UUU-rps16_p3	trnK-UUU-rps16	3	CCGCACTTAAAAGCCGAGTA	TATTTCAAAGAAGGCGGGGG	20	20	50.0	50.0	58.3	57.9	5.3	0.0	0.420616	4541	5587	1149-1183	1.000000	80.1
trnK-UUU-rps16_p4	trnK-UUU-rps16	4	AAAGCCGAGTACTCTACCGT	AGAAGGCGGGGGTTTTTATG	20	20	50.0	50.0	58.2	58.2	20.3	0.0	0.321091	4541	5587	1132-1162	0.500000	59.1
trnK-UUU-rps16_p5	trnK-UUU-rps16	5	AAGCCGAGTACTCTACCGTT	AGAAGGCGGGGGTTTTTATG	20	20	50.0	50.0	58.2	58.2	20.3	0.0	0.321091	4541	5587	1131-1161	0.500000	59.1
trnS-GCU-trnG-UCC_p1	trnS-GCU-trnG-UCC	1	CAATCCGACGCTTTAGTCCA	ACGAATCACACTTTTACCACT	20	21	50.0	38.1	58.0	55.5	0.0	0.0	3.540425	9237	9945	770-802	1.000000	58.1
trnS-GCU-trnG-UCC_p2	trnS-GCU-trnG-UCC	2	AATCCGACGCTTTAGTCCAC	ACGAATCACACTTTTACCACT	20	21	50.0	38.1	58.0	55.5	0.0	0.0	3.541813	9237	9945	769-801	1.000000	58.1
trnS-GCU-trnG-UCC_p3	trnS-GCU-trnG-UCC	3	CAATCCGACGCTTTAGTCCA	AGAACGAATCACACTTTTACCAC	20	23	50.0	39.1	58.0	57.3	0.0	0.0	3.673141	9237	9945	773-805	1.000000	57.1
trnS-GCU-trnG-UCC_p4	trnS-GCU-trnG-UCC	4	CAATCCGACGCTTTAGTCCA	GAACGAATCACACTTTTACCACT	20	23	50.0	39.1	58.0	57.3	0.0	0.0	3.673141	9237	9945	772-804	1.000000	57.1
trnS-GCU-trnG-UCC_p5	trnS-GCU-trnG-UCC	5	AATCCGACGCTTTAGTCCAC	GAACGAATCACACTTTTACCACT	20	23	50.0	39.1	58.0	57.3	0.0	0.0	3.674529	9237	9945	771-803	1.000000	57.0
rpoC2_p1	rpoC2	1	TTCTCCCGTTTTCGTCCATT	TGGGTCCATTCAACGTCAAC	20	20	45.0	50.0	57.4	58.4	0.0	0.0	0.954347	19086	19205	243-243	1.000000	79.3
rpoC2_p2	rpoC2	2	AATTCTCCCGTTTTCGTCCA	TGGGTCCATTCAACGTCAAC	20	20	45.0	50.0	57.4	58.4	0.0	0.0	0.954347	19086	19205	245-245	1.000000	79.2
rpoC2_p3	rpoC2	3	TTCTCCCGTTTTCGTCCATT	GGTCCATTCAACGTCAACGA	20	20	45.0	50.0	57.4	58.5	0.0	9.9	1.050011	19086	19205	241-241	1.000000	78.6
rpoC2_p4	rpoC2	4	AATTCTCCCGTTTTCGTCCA	GGTCCATTCAACGTCAACGA	20	20	45.0	50.0	57.4	58.5	0.0	9.9	1.050011	19086	19205	243-243	1.000000	78.5
rpoC2_p5	rpoC2	5	TTCTCCCGTTTTCGTCCATT	ATGGGTCCATTCAACGTCAA	20	20	45.0	45.0	57.4	57.4	0.0	0.0	1.131976	19086	19205	244-244	1.000000	77.8
psbC-trnS-UGA_p1	psbC-trnS-UGA	1	AGCTGCAGCAGGATTTGAAA	GGTTGATAGCTCCGGTCTTG	20	20	45.0	55.0	58.4	58.1	38.2	0.0	0.434339	37984	38236	403-414	1.000000	80.1
psbC-trnS-UGA_p2	psbC-trnS-UGA	2	GCTGCAGCAGGATTTGAAAA	GGTTGATAGCTCCGGTCTTG	20	20	45.0	55.0	57.6	58.1	33.4	0.0	0.502562	37984	38236	402-413	1.000000	79.5
psbC-trnS-UGA_p3	psbC-trnS-UGA	3	TAGGTCATTTATGGCACGCG	GGTTGATAGCTCCGGTCTTG	20	20	50.0	55.0	58.4	58.1	3.5	0.0	0.481207	37984	38236	437-448	1.000000	79.4
psbC-trnS-UGA_p4	psbC-trnS-UGA	4	AGCTGCAGCAGGATTTGAAA	GATGGCCGAGTGGTTGATAG	20	20	45.0	55.0	58.4	58.1	38.2	0.0	0.504584	37984	38236	414-425	1.000000	79.4
psbC-trnS-UGA_p5	psbC-trnS-UGA	5	TAGGTCATTTATGGCACGCG	GATGGCCGAGTGGTTGATAG	20	20	50.0	55.0	58.4	58.1	3.5	0.0	0.551451	37984	38236	448-459	1.000000	78.8
ycf3-trnS-GGA_p1	ycf3-trnS-GGA	1	CTCTTTCTCGCCCGAAGTTG	AGGTGAAGGGTACGGAAAGA	20	20	55.0	50.0	58.9	58.0	0.0	0.0	0.948202	47403	48128	916-928	1.000000	75.6
ycf3-trnS-GGA_p2	ycf3-trnS-GGA	2	CTCTTTCTCGCCCGAAGTTG	CCCTCGGTAAACAAAAGCCT	20	20	55.0	50.0	58.9	58.1	0.0	0.0	1.022757	47403	48128	884-896	1.000000	75.1
ycf3-trnS-GGA_p3	ycf3-trnS-GGA	3	CTCTTTCTCGCCCGAAGTTG	ACGGAAAGAGAGGGATTCGA	20	20	55.0	50.0	58.9	58.2	0.0	0.0	1.079743	47403	48128	905-917	1.000000	74.6
ycf3-trnS-GGA_p4	ycf3-trnS-GGA	4	CTTTCTCGCCCGAAGTTGT	AGGTGAAGGGTACGGAAAGA	19	20	52.6	50.0	58.1	58.0	0.0	0.0	1.105428	47403	48128	914-926	1.000000	74.4
ycf3-trnS-GGA_p5	ycf3-trnS-GGA	5	TTTCTCGCCCGAAGTTGTC	AGGTGAAGGGTACGGAAAGA	19	20	52.6	50.0	58.1	58.0	0.0	0.0	1.110991	47403	48128	913-925	1.000000	74.3
trnT-UGU-trnL-UAA_p1	trnT-UGU-trnL-UAA	1	CGATGACCCTCGCATTACAA	ACCAATTTCGCCATATCCCC	20	20	50.0	50.0	58.1	58.0	0.0	0.0	0.070035	49699	50967	1280-1399	1.000000	86.6
trnT-UGU-trnL-UAA_p2	trnT-UGU-trnL-UAA	2	CGATGACCCTCGCATTACAA	TAGCGTCTACCAATTTCGCC	20	20	50.0	50.0	58.1	58.1	0.0	0.0	0.134844	49699	50967	1288-1407	1.000000	86.1
trnT-UGU-trnL-UAA_p3	trnT-UGU-trnL-UAA	3	GCGATGCTCTAACCTCTGAG	ACCAATTTCGCCATATCCCC	20	20	55.0	50.0	57.9	58.0	0.0	0.0	0.137364	49699	50967	1258-1377	1.000000	86.1
trnT-UGU-trnL-UAA_p4	trnT-UGU-trnL-UAA	4	GCGATGCTCTAACCTCTGAG	TAGCGTCTACCAATTTCGCC	20	20	55.0	50.0	57.9	58.1	0.0	0.0	0.202173	49699	50967	1266-1385	1.000000	85.6
trnT-UGU-trnL-UAA_p5	trnT-UGU-trnL-UAA	5	AAAGCCAACTATCGGAGTCG	ACCAATTTCGCCATATCCCC	20	20	50.0	50.0	57.7	58.0	0.0	0.0	0.299877	49699	50967	1303-1422	1.000000	84.7
trnF-GAA-ndhJ_p1	trnF-GAA-ndhJ	1	GGGATAGCTCAGCTGGTAGA	GAAAGCTGGATAGGATGGCC	20	20	55.0	55.0	58.0	58.1	10.3	0.0	0.099567	51999	52670	811-819	1.000000	81.6
trnF-GAA-ndhJ_p2	trnF-GAA-ndhJ	2	CCTCGTGTCACCAGTTCAAA	GAAAGCTGGATAGGATGGCC	20	20	50.0	55.0	58.1	58.1	0.0	0.0	0.148958	51999	52670	775-783	1.000000	81.3
trnF-GAA-ndhJ_p3	trnF-GAA-ndhJ	3	TGATGCGTAAGGAATGGTCG	GAAAGCTGGATAGGATGGCC	20	20	50.0	55.0	58.1	58.1	0.0	0.0	0.162473	51999	52670	830-838	1.000000	81.0
trnF-GAA-ndhJ_p4	trnF-GAA-ndhJ	4	CTGGTAGAGCAGAGGACTGA	GAAAGCTGGATAGGATGGCC	20	20	55.0	55.0	57.9	58.1	2.5	0.0	0.233360	51999	52670	799-807	1.000000	80.5
trnF-GAA-ndhJ_p5	trnF-GAA-ndhJ	5	AAGGAATGGTCGGGATAGCT	GAAAGCTGGATAGGATGGCC	20	20	50.0	55.0	58.2	58.1	0.0	0.0	0.289248	51999	52670	822-830	1.000000	80.0
rpl22_p1	rpl22	1	TGCACCAAGTCTGAAACCAA	TCCCGAGCGTCTACCATTAT	20	20	45.0	50.0	57.9	58.0	0.0	0.0	0.145177	88020	88397	835-853	1.000000	85.1
rpl22_p2	rpl22	2	AGCCCTAATGGTTTGTAGCG	TCCCGAGCGTCTACCATTAT	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.047266	88020	88397	1360-1378	1.000000	84.2
rpl22_p3	rpl22	3	AGCCCTAATGGTTTGTAGCG	GGGCCCCAATTGGTAGAAAA	20	20	50.0	50.0	58.0	58.1	0.0	22.9	0.092807	88020	88397	1651-1670	1.000000	83.5
rpl22_p4	rpl22	4	AGCCCTAATGGTTTGTAGCG	TTGATTCTTCGTCGCCGTAG	20	20	50.0	50.0	58.0	58.1	0.0	0.0	0.134243	88020	88397	1559-1578	1.000000	83.3
rpl22_p5	rpl22	5	AGCCCTAATGGTTTGTAGCG	AAAAGTTTGGGTAGAGCCGG	20	20	50.0	50.0	58.0	58.1	0.0	0.0	0.132749	88020	88397	1757-1776	1.000000	82.9
rpl22-rps19_p1	rpl22-rps19	1	TGCACCAAGTCTGAAACCAA	TCCCGAGCGTCTACCATTAT	20	20	45.0	50.0	57.9	58.0	0.0	0.0	0.145177	88398	88512	835-853	1.000000	85.1
rpl22-rps19_p2	rpl22-rps19	2	TTGCACCAAGTCTGAAACCA	TCCCGAGCGTCTACCATTAT	20	20	45.0	50.0	57.9	58.0	0.0	0.0	0.145177	88398	88512	836-854	1.000000	85.1
rpl22-rps19_p3	rpl22-rps19	3	CCGTTGACTTCCGCTTTACT	TCCCGAGCGTCTACCATTAT	20	20	50.0	50.0	58.2	58.0	0.0	0.0	0.216873	88398	88512	536-555	1.000000	84.2
rpl22-rps19_p4	rpl22-rps19	4	TGCACCAAGTCTGAAACCAA	GGGCCCCAATTGGTAGAAAA	20	20	45.0	50.0	57.9	58.1	0.0	22.9	0.190719	88398	88512	1126-1145	1.000000	83.8
rpl22-rps19_p5	rpl22-rps19	5	TTGCACCAAGTCTGAAACCA	GGGCCCCAATTGGTAGAAAA	20	20	45.0	50.0	57.9	58.1	0.0	22.9	0.190719	88398	88512	1127-1146	1.000000	83.8
ndhF_p1	ndhF	1	ACAGGAACAAGAGGGATCCA	TGGAGATCAGAGCCTTTCCT	20	20	50.0	50.0	58.0	57.7	15.2	0.0	0.305431	114484	116760	2988-3000	1.000000	80.4
ndhF_p2	ndhF	2	AAGAGGGATCCACCGAAGAA	TGGAGATCAGAGCCTTTCCT	20	20	50.0	50.0	58.0	57.7	15.2	0.0	0.308017	114484	116760	2980-2992	1.000000	80.4
ndhF_p3	ndhF	3	AGGGATCCACCGAAGAAGAT	TGGAGATCAGAGCCTTTCCT	20	20	50.0	50.0	57.8	57.7	15.2	0.0	0.443154	114484	116760	2977-2989	1.000000	79.3
ndhF_p4	ndhF	4	CAGGAACAAGAGGGATCCAC	TGGAGATCAGAGCCTTTCCT	20	20	55.0	50.0	57.6	57.7	15.2	0.0	0.670587	114484	116760	2987-2999	1.000000	77.5
ndhF_p5	ndhF	5	AGAGGGATCCACCGAAGAAG	TGGAGATCAGAGCCTTTCCT	20	20	55.0	50.0	58.5	57.7	15.2	0.0	0.770065	114484	116760	2979-2991	1.000000	76.7
ndhF-rpl32_p1	ndhF-rpl32	1	GCTGCAGTAGATCGTGTGAA	AAAAGCTTTCAATGCTGCCC	20	20	50.0	45.0	58.0	57.8	16.6	13.1	0.178072	116761	118083	1924-2026	1.000000	82.4
ndhF-rpl32_p2	ndhF-rpl32	2	GCTGCAGTAGATCGTGTGAA	CTACGTTTCGAGCCAGAACA	20	20	50.0	50.0	58.0	57.9	16.6	0.0	0.064034	116761	118083	2030-2076	0.625000	68.0
ndhF-rpl32_p3	ndhF-rpl32	3	GCTGCAGTAGATCGTGTGAA	ACTACGTTTCGAGCCAGAAC	20	20	50.0	50.0	58.0	57.9	16.6	0.0	0.065448	116761	118083	2031-2077	0.625000	68.0
ndhF-rpl32_p4	ndhF-rpl32	4	AATCTCCGACGCGATTAGTG	CTACGTTTCGAGCCAGAACA	20	20	50.0	50.0	57.9	57.9	15.9	0.0	0.181442	116761	118083	2074-2120	0.625000	67.1
ndhF-rpl32_p5	ndhF-rpl32	5	AATCTCCGACGCGATTAGTG	ACTACGTTTCGAGCCAGAAC	20	20	50.0	50.0	57.9	57.9	15.9	0.0	0.182856	116761	118083	2075-2121	0.625000	67.1
rpl32-trnL-UAG_p1	rpl32-trnL-UAG	1	GGGCAGCATTGAAAGCTTTT	AGCGTGTCTACCGATTTCAC	20	20	45.0	50.0	57.8	58.0	14.0	0.0	0.182194	118243	119958	1814-1907	1.000000	87.0
rpl32-trnL-UAG_p2	rpl32-trnL-UAG	2	GGGCAGCATTGAAAGCTTTT	CAGCGTGTCTACCGATTTCA	20	20	45.0	50.0	57.8	58.0	14.0	0.0	0.183564	118243	119958	1815-1908	1.000000	87.0
rpl32-trnL-UAG_p3	rpl32-trnL-UAG	3	GGGCAGCATTGAAAGCTTTT	TAGGAGATATCATGCCGCCA	20	20	45.0	50.0	57.8	58.1	14.0	5.3	0.237172	118243	119958	1877-1970	1.000000	86.4
rpl32-trnL-UAG_p4	rpl32-trnL-UAG	4	GGGCAGCATTGAAAGCTTTT	TCCTAAGAGCAGCGTGTCTA	20	20	45.0	50.0	57.8	57.9	14.0	0.0	0.298641	118243	119958	1824-1917	1.000000	86.0
rpl32-trnL-UAG_p5	rpl32-trnL-UAG	5	GGGCAGCATTGAAAGCTTTT	CTAAGAGCAGCGTGTCTACC	20	20	45.0	55.0	57.8	57.8	14.0	0.0	0.370658	118243	119958	1822-1915	1.000000	85.4
ccsA-ndhD_p1	ccsA-ndhD	1	CCCTGGGCGAGATTCAATAC	TACAGGACTCAATGGAGCCA	20	20	55.0	50.0	58.1	58.0	0.0	11.7	0.164555	121112	121404	1230-1239	1.000000	81.6
ccsA-ndhD_p2	ccsA-ndhD	2	GGGAGCAGTATGGGCTAATG	TACAGGACTCAATGGAGCCA	20	20	55.0	50.0	57.8	58.0	0.0	11.7	0.239918	121112	121404	1064-1073	1.000000	81.2
ccsA-ndhD_p3	ccsA-ndhD	3	TCCCTGGGCGAGATTCAATA	TACAGGACTCAATGGAGCCA	20	20	50.0	50.0	58.2	58.0	8.2	11.7	0.247548	121112	121404	1231-1240	1.000000	80.9
ccsA-ndhD_p4	ccsA-ndhD	4	AATGAGTCGTTTTCCCTGGG	TACAGGACTCAATGGAGCCA	20	20	50.0	50.0	57.8	58.0	6.3	11.7	0.249163	121112	121404	1243-1252	1.000000	80.9
ccsA-ndhD_p5	ccsA-ndhD	5	TACCAGCTCTCCAATCCCAT	TACAGGACTCAATGGAGCCA	20	20	50.0	50.0	57.8	58.0	0.0	11.7	0.245533	121112	121404	1398-1407	0.875000	75.5
ycf1_p1	ycf1	1	TGCCAAGGTTTCAGACGAAA	CGTACCCACCCAGTTTGTTA	20	20	45.0	50.0	58.0	57.5	0.0	0.0	0.592861	133438	133557	1459-1540	1.000000	79.3
ycf1_p2	ycf1	2	TGCCAAGGTTTCAGACGAAA	TTACGTACCCACCCAGTTTG	20	20	45.0	50.0	58.0	57.5	0.0	0.0	0.592861	133438	133557	1462-1543	1.000000	79.3
ycf1_p3	ycf1	3	TGCCAAGGTTTCAGACGAAA	TACGTACCCACCCAGTTTGT	20	20	45.0	50.0	58.0	58.6	0.0	0.0	0.623030	133438	133557	1461-1542	1.000000	79.1
ycf1_p4	ycf1	4	ATCCTCCGACCATTCAGGAA	CGTACCCACCCAGTTTGTTA	20	20	50.0	50.0	58.1	57.5	0.0	0.0	0.667724	133438	133557	1319-1400	1.000000	79.0
ycf1_p5	ycf1	5	ATCCTCCGACCATTCAGGAA	TTACGTACCCACCCAGTTTG	20	20	50.0	50.0	58.1	57.5	0.0	0.0	0.667724	133438	133557	1322-1403	1.000000	79.0