Hotspot

rpl32-trnL-UAG

SSC · 108393-108916 · 524 bp

Back to Arthraxon

Pi 0.0126

MarkerSeek score 61.00

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: SSC
length_bp: 524
variable_sites: 16
pi: 0.0126
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	CAACGAGCATCCAAAACCAA	TTCGAACCGAGATGCTTGAG	45.0	50.0	57.2	58.0	623-642 bp	1.0	78.7
rpl32-trnL-UAG_p2	CAACGAGCATCCAAAACCAA	GGATTCGAACCGAGATGCTT	45.0	50.0	57.2	58.1	626-645 bp	1.0	78.2
rpl32-trnL-UAG_p3	CAACGAGCATCCAAAACCAA	TCCTAAGAGCAGCGTGTCTA	45.0	50.0	57.2	57.9	596-615 bp	1.0	77.9
rpl32-trnL-UAG_p4	CAACGAGCATCCAAAACCAA	AGCAGCGTGTCTACCAATTT	45.0	45.0	57.2	57.8	589-608 bp	1.0	77.5
rpl32-trnL-UAG_p5	CAACGAGCATCCAAAACCAA	GCTTGAGCACTGCTTCCTAA	45.0	50.0	57.2	58.2	610-629 bp	1.0	77.3