Hotspot

ndhF-rpl32

SSC · 107373-108212 · 840 bp

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Pi 0.00595
MarkerSeek score 53.50
Primer available yes
Label ndhF-rpl32

Feature Metadata

label_name
ndhF-rpl32
feature_type
igs
region
SSC
length_bp
840
variable_sites
12
pi
0.00595
unique_haplotype_count
5
primer_available
yes
alignment_reliability
1.00
markerseek_score
53.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF-rpl32_p1 CCAGGCATATTGATATGTATGTTCC TGACATCGAAGTACGTTTTT 40.0 35.0 57.7 53.2 878-900 bp 1.0 43.1
ndhF-rpl32_p2 CCAGGCATATTGATATGTATGTTCC TTGACATCGAAGTACGTTTT 40.0 35.0 57.7 53.2 879-901 bp 1.0 43.1
ndhF-rpl32_p3 CCAGGCATATTGATATGTATGTTCC TTTGACATCGAAGTACGTTT 40.0 35.0 57.7 53.2 880-902 bp 1.0 43.1
ndhF-rpl32_p4 CCAGGCATATTGATATGTATGTTCC TTTTGACATCGAAGTACGTT 40.0 35.0 57.7 53.2 881-903 bp 1.0 43.1
ndhF-rpl32_p5 CCAGGCATATTGATATGTATGTTCC TTTTTGACATCGAAGTACGT 40.0 35.0 57.7 53.2 882-904 bp 1.0 43.1