Hotspot

ndhC-trnV-UAC

LSC · 52464-53372 · 909 bp

Back to Arthraxon

Pi 0.0155

MarkerSeek score 64.20

Primer available yes

Label ndhC-trnV-UAC

Feature Metadata

label_name: ndhC-trnV-UAC
feature_type: igs
region: LSC
length_bp: 909
variable_sites: 33
pi: 0.0155
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 64.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-trnV-UAC_p1	ACCAAAATAGGAATAAGGCTTGC	GGTTCGAATCCGTATAGCCC	39.1	55.0	57.4	57.9	991-1041 bp	1.0	55.2
ndhC-trnV-UAC_p2	ACCAAAATAGGAATAAGGCTTGC	AGCCCAATGCAGAAAAATGC	39.1	45.0	57.4	57.9	1084-1134 bp	1.0	54.6
ndhC-trnV-UAC_p3	ACCAAAATAGGAATAAGGCTTGC	CGGTTCGAATCCGTATAGCC	39.1	55.0	57.4	58.3	992-1042 bp	1.0	53.4
ndhC-trnV-UAC_p4	CCAAAATAGGAATAAGGCTTGCT	GGTTCGAATCCGTATAGCCC	39.1	55.0	57.1	57.9	990-1040 bp	1.0	53.1
ndhC-trnV-UAC_p5	TCATATTCGTGAAGCAGAAACA	GGTTCGAATCCGTATAGCCC	36.4	55.0	56.1	57.9	943-993 bp	1.0	53.1