Hotspot

trnD-GUC-psbM

LSC · 17498-18462 · 965 bp

Back to Arthraxon

Pi 0.009

MarkerSeek score 61.10

Primer available yes

Label trnD-GUC-psbM

Feature Metadata

label_name: trnD-GUC-psbM
feature_type: igs
region: LSC
length_bp: 965
variable_sites: 19
pi: 0.009
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnD-GUC-psbM_p1	AATAGGCATGCCATACACCC	TGCGAGAATATTGACTTCCA	50.0	40.0	57.7	54.5	1071-1105 bp	1.0	52.7
trnD-GUC-psbM_p2	TTCAATTGGTCAGAGCACCG	TGCGAGAATATTGACTTCCA	50.0	40.0	58.5	54.5	1037-1071 bp	1.0	51.3
trnD-GUC-psbM_p3	AATAGGCATGCCATACACCC	ATGCGAGAATATTGACTTCCA	50.0	38.1	57.7	55.1	1072-1106 bp	1.0	49.6
trnD-GUC-psbM_p4	AATAGGCATGCCATACACCC	TGCGAGAATATTGACTTCCAT	50.0	38.1	57.7	55.1	1071-1105 bp	1.0	49.6
trnD-GUC-psbM_p5	AATAGGCATGCCATACACCC	AGAATGAACAATGCTGTAGC	50.0	40.0	57.7	54.0	1097-1131 bp	1.0	48.6