Hotspot

trnT-GGU-trnE-UUC

LSC · 16097-16975 · 879 bp

Back to Arthraxon

Pi 0.0131

MarkerSeek score 65.40

Primer available yes

Label trnT-GGU-trnE-UUC

Feature Metadata

label_name: trnT-GGU-trnE-UUC
feature_type: igs
region: LSC
length_bp: 879
variable_sites: 27
pi: 0.0131
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 65.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-GGU-trnE-UUC_p1	ACCACTAAGTGAAAAGCCCT	GCTGCCTCCTTGAAAGAGAG	45.0	55.0	56.7	58.3	960-1013 bp	1.0	73.3
trnT-GGU-trnE-UUC_p2	ACCACTAAGTGAAAAGCCCT	TGTCCTGAACCACTAGACGA	45.0	50.0	56.7	57.7	939-992 bp	1.0	73.2
trnT-GGU-trnE-UUC_p3	TGAACCGATGACTTATGCCT	GCTGCCTCCTTGAAAGAGAG	45.0	55.0	56.6	58.3	930-983 bp	1.0	73.2
trnT-GGU-trnE-UUC_p4	TGAACCGATGACTTATGCCT	TGTCCTGAACCACTAGACGA	45.0	50.0	56.6	57.7	909-962 bp	1.0	73.1
trnT-GGU-trnE-UUC_p5	CCGATGACTTATGCCTTACCA	GCTGCCTCCTTGAAAGAGAG	47.6	55.0	57.5	58.3	926-979 bp	1.0	72.4