Hotspot

psbK-psbI

LSC · 7704-8111 · 408 bp

Back to Arthraxon

Pi 0.0137

MarkerSeek score 54.70

Primer available yes

Label psbK-psbI

Feature Metadata

label_name: psbK-psbI
feature_type: igs
region: LSC
length_bp: 408
variable_sites: 13
pi: 0.0137
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 54.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbK-psbI_p1	CGTGGATGTTATGCCTGTCA	TTTTTACTCCTCACGCCCAG	50.0	50.0	58.0	57.8	599-607 bp	1.0	80.9
psbK-psbI_p2	GTTTGGCAAGCTGCTGTAAG	TTTTTACTCCTCACGCCCAG	50.0	50.0	57.9	57.8	550-558 bp	1.0	80.9
psbK-psbI_p3	TTAGCGTTTGTTTGGCAAGC	TTTTTACTCCTCACGCCCAG	45.0	50.0	58.2	57.8	559-567 bp	1.0	80.0
psbK-psbI_p4	ACCCGAAGCTTATGCCATTT	TTTTTACTCCTCACGCCCAG	45.0	50.0	57.9	57.8	629-637 bp	1.0	79.8
psbK-psbI_p5	TGGCAAGCTGCTGTAAGTTT	TTTTTACTCCTCACGCCCAG	45.0	50.0	58.3	57.8	547-555 bp	1.0	78.9