Hotspot

rpl16

LSC · 79970-81403 · 1434 bp

Back to Artemisia

Pi 0.00613

MarkerSeek score 47.10

Primer available yes

Label rpl16

Feature Metadata

label_name: rpl16
feature_type: gene
region: LSC
length_bp: 1434
variable_sites: 80
pi: 0.00613
unique_haplotype_count: 59
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 47.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl16_p1	CGCACCAAAAACTCGAGTTC	GCAAATTGCAGGACGTATCG	50.0	50.0	58.0	57.9	2154-2242 bp	0.992063	87.4
rpl16_p2	ACACATCAATTCTCGAGCCC	GCAAATTGCAGGACGTATCG	50.0	50.0	58.0	57.9	1914-2002 bp	0.992063	87.4
rpl16_p3	GCGTCTTTGATCACAGCAAC	GCAAATTGCAGGACGTATCG	50.0	50.0	58.0	57.9	1988-2076 bp	0.992063	87.4
rpl16_p4	ACTCGAACCACGCTTTTTCT	GCAAATTGCAGGACGTATCG	45.0	50.0	58.1	57.9	2479-2574 bp	0.992063	87.2
rpl16_p5	TCGACGATTGCTAGCTCCTA	GCAAATTGCAGGACGTATCG	50.0	50.0	58.0	57.9	1944-2032 bp	0.984127	87.1