Hotspot

rpl32-trnL-UAG

SSC · 111327-112204 · 878 bp

Back to Artemisia

Pi 0.0108

MarkerSeek score 74.20

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: SSC
length_bp: 878
variable_sites: 125
pi: 0.0108
unique_haplotype_count: 94
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 74.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	GGCGGCATTAAAAGCTTTGT	AGCGTGTCTACCGATTTCAC	45.0	50.0	57.9	58.0	837-1025 bp	1.0	91.3
rpl32-trnL-UAG_p2	GGCGGCATTAAAAGCTTTGT	CAGCGTGTCTACCGATTTCA	45.0	50.0	57.9	58.0	838-1026 bp	1.0	91.3
rpl32-trnL-UAG_p3	AAAGGATATTGGGCGGCATT	AGCGTGTCTACCGATTTCAC	45.0	50.0	57.9	58.0	848-1036 bp	1.0	91.2
rpl32-trnL-UAG_p4	AAAGGATATTGGGCGGCATT	CAGCGTGTCTACCGATTTCA	45.0	50.0	57.9	58.0	849-1037 bp	1.0	91.2
rpl32-trnL-UAG_p5	GGCGGCATTAAAAGCTTTGT	TCCTAAGAGCAGCGTGTCTA	45.0	50.0	57.9	57.9	847-1035 bp	1.0	90.4