Hotspot

ndhF-rpl32

SSC · 110159-111161 · 1003 bp

Back to Artemisia

Pi 0.00837
MarkerSeek score 71.40
Primer available yes
Label ndhF-rpl32

Feature Metadata

label_name
ndhF-rpl32
feature_type
igs
region
SSC
length_bp
1003
variable_sites
102
pi
0.00837
unique_haplotype_count
82
primer_available
yes
alignment_reliability
0.99
markerseek_score
71.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF-rpl32_p1 CTTTTTGTTGCCGTCGGAAA ACAAAGCTTTTAATGCCGCC 45.0 45.0 57.8 57.9 1172-1221 bp 1.0 89.0
ndhF-rpl32_p2 CTTTTTGTTGCCGTCGGAAA AATGCCGCCCAATATCCTTT 45.0 45.0 57.8 57.9 1161-1210 bp 1.0 89.0
ndhF-rpl32_p3 AAGACTTTTTGTTGCCGTCG ACAAAGCTTTTAATGCCGCC 45.0 45.0 57.5 57.9 1176-1225 bp 0.992063 86.1
ndhF-rpl32_p4 ACGAAGACTTTTTGTTGCCG ACAAAGCTTTTAATGCCGCC 45.0 45.0 57.5 57.9 1179-1228 bp 0.992063 86.1
ndhF-rpl32_p5 CGAAGACTTTTTGTTGCCGT ACAAAGCTTTTAATGCCGCC 45.0 45.0 57.5 57.9 1178-1227 bp 0.992063 86.1