Hotspot

rpl16-rps3

LSC · 81404-81562 · 159 bp

Back to Artemisia

Pi 0.0152

MarkerSeek score 61.30

Primer available yes

Label rpl16-rps3

Feature Metadata

label_name: rpl16-rps3
feature_type: igs
region: LSC
length_bp: 159
variable_sites: 17
pi: 0.0152
unique_haplotype_count: 29
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl16-rps3_p1	AAACCAACGAGTCACACACT	GAGGGTAGGGTTCCACTACA	45.0	55.0	57.6	57.8	294-320 bp	1.0	83.9
rpl16-rps3_p2	AAACCAACGAGTCACACACT	AGAGGGTAGGGTTCCACTAC	45.0	55.0	57.6	57.5	295-321 bp	1.0	81.5
rpl16-rps3_p3	AACGAGTCACACACTAAGCA	GAGGGTAGGGTTCCACTACA	45.0	55.0	57.1	57.8	289-315 bp	1.0	80.2
rpl16-rps3_p4	AAACCAACGAGTCACACACT	AGGGTAGGGTTCCACTACAA	45.0	50.0	57.6	57.3	293-319 bp	1.0	80.1
rpl16-rps3_p5	AAACCAACGAGTCACACACT	GAGAGGGTAGGGTTCCACTA	45.0	55.0	57.6	57.2	296-322 bp	1.0	79.1