Hotspot

psaA-ycf3

LSC · 41174-41889 · 716 bp

Back to Artemisia

Pi 0.00546

MarkerSeek score 66.00

Primer available yes

Label psaA-ycf3

Feature Metadata

label_name: psaA-ycf3
feature_type: igs
region: LSC
length_bp: 716
variable_sites: 44
pi: 0.00546
unique_haplotype_count: 84
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 66.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaA-ycf3_p1	GAGAAATGACCCGGTCTAGC	CAAGCCGCTGAGTATTGGAA	55.0	50.0	57.8	58.3	898-936 bp	1.0	84.1
psaA-ycf3_p2	GGGCCTTTCGCTATTGTTCT	CAAGCCGCTGAGTATTGGAA	50.0	50.0	58.2	58.3	919-957 bp	1.0	83.9
psaA-ycf3_p3	GAGAAATGACCCGGTCTAGC	AGCCGCTGAGTATTGGAAAC	55.0	50.0	57.8	58.3	896-934 bp	0.992063	83.6
psaA-ycf3_p4	GGGCCTTTCGCTATTGTTCT	AGCCGCTGAGTATTGGAAAC	50.0	50.0	58.2	58.3	917-955 bp	0.992063	83.4
psaA-ycf3_p5	GAGAAATGACCCGGTCTAGC	AAGCCGCTGAGTATTGGAAA	55.0	45.0	57.8	57.5	897-935 bp	0.992063	81.7