Hotspot

atpI-atpH

LSC · 24919-26045 · 1127 bp

Back to Artemisia

Pi 0.00598

MarkerSeek score 65.10

Primer available yes

Label atpI-atpH

Feature Metadata

label_name: atpI-atpH
feature_type: igs
region: LSC
length_bp: 1127
variable_sites: 76
pi: 0.00598
unique_haplotype_count: 73
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 65.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpI-atpH_p1	TCCCTGTCATGTTCCTTGGA	ATAACGGAAGCGGCAGAAAT	50.0	45.0	58.3	58.0	1177-1284 bp	1.0	86.5
atpI-atpH_p2	TCCCTGTCATGTTCCTTGGA	TACCTTGACCAACTCCAGGT	50.0	50.0	58.3	57.9	1233-1340 bp	1.0	85.7
atpI-atpH_p3	TCCCTGTCATGTTCCTTGGA	AGCCAATCCAGCAGCAATAA	50.0	45.0	58.3	57.9	1193-1300 bp	1.0	85.6
atpI-atpH_p4	TCCCTGTCATGTTCCTTGGA	AATAACGGAAGCGGCAGAAA	50.0	45.0	58.3	58.2	1178-1285 bp	1.0	85.4
atpI-atpH_p5	TCCCTGTCATGTTCCTTGGA	GCAGTACCTTGACCAACTCC	50.0	55.0	58.3	58.2	1237-1344 bp	1.0	85.1