Hotspot

rps16-trnQ-UUG

LSC · 6298-7197 · 900 bp

Back to Artemisia

Pi 0.00795

MarkerSeek score 66.10

Primer available yes

Label rps16-trnQ-UUG

Feature Metadata

label_name: rps16-trnQ-UUG
feature_type: igs
region: LSC
length_bp: 900
variable_sites: 88
pi: 0.00795
unique_haplotype_count: 62
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 66.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16-trnQ-UUG_p1	AAGTCGCACGTTGCTTTCTA	GAGGTTCGAATCCTTCCGTC	45.0	55.0	58.1	58.1	952-1002 bp	1.0	87.8
rps16-trnQ-UUG_p2	TCATGTCCTTCAAGTCGCAC	GAGGTTCGAATCCTTCCGTC	50.0	55.0	58.2	58.1	963-1013 bp	1.0	87.2
rps16-trnQ-UUG_p3	CACACCGGATCATGTCCTTC	GAGGTTCGAATCCTTCCGTC	55.0	55.0	58.3	58.1	972-1022 bp	1.0	86.1
rps16-trnQ-UUG_p4	CACCGGATCATGTCCTTCAA	GAGGTTCGAATCCTTCCGTC	50.0	55.0	57.6	58.1	970-1020 bp	1.0	85.7
rps16-trnQ-UUG_p5	CCAAGAGCACCTTCATTCCT	GAGGTTCGAATCCTTCCGTC	50.0	55.0	57.5	58.1	1023-1073 bp	0.97619	84.0