Hotspot

rps18

LSC · 73704-74273 · 570 bp

Back to Artabotrys

Pi 0.0109

MarkerSeek score 61.90

Primer available yes

Label rps18

Feature Metadata

label_name: rps18
feature_type: gene
region: LSC
length_bp: 570
variable_sites: 6
pi: 0.0109
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 61.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps18_p1	TCGGATCCGAAATGCATGAA	TGATTCAATTGAGGAGTAGGCC	45.0	45.5	57.7	57.8	649-670 bp	1.0	63.4
rps18_p2	TCGGATCCGAAATGCATGAA	TTCAATTGAGGAGTAGGCCT	45.0	45.0	57.7	55.8	646-667 bp	1.0	63.3
rps18_p3	GTCCTATTTCGGTCGGATCC	TGATTCAATTGAGGAGTAGGCC	55.0	45.5	57.6	57.8	661-682 bp	1.0	62.5
rps18_p4	GTCCTATTTCGGTCGGATCC	TTCAATTGAGGAGTAGGCCT	55.0	45.0	57.6	55.8	658-679 bp	1.0	62.3
rps18_p5	TCGGATCCGAAATGCATGAA	GATTCAATTGAGGAGTAGGCCT	45.0	45.5	57.7	57.6	648-669 bp	1.0	61.2