Hotspot

ndhF

SSC · 133123-135348 · 2226 bp

Back to Artabotrys

Pi 0.456

MarkerSeek score 76.00

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: SSC
length_bp: 2226
variable_sites: 745
pi: 0.456
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.73
markerseek_score: 76.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	CATAAAGGGAGCGTCGTTCA	CCGTCTAGAGTATACCGGGG	50.0	60.0	58.0	58.2	3000 bp	0.5	58.5
ndhF_p2	AAAGGGAGCGTCGTTCATAC	CCGTCTAGAGTATACCGGGG	50.0	60.0	58.0	58.2	2997 bp	0.5	58.5
ndhF_p3	GGAGCGTCGTTCATACTTGT	CCGTCTAGAGTATACCGGGG	50.0	60.0	58.0	58.2	2993 bp	0.5	58.5
ndhF_p4	CATAAAGGGAGCGTCGTTCA	GAACTGGGGAATCGGAAACA	50.0	50.0	58.0	57.8	2587 bp	0.5	58.4
ndhF_p5	AAAGGGAGCGTCGTTCATAC	GAACTGGGGAATCGGAAACA	50.0	50.0	58.0	57.8	2584 bp	0.5	58.4