Hotspot

ndhA-ndhI

IRb · 126458-126534 · 77 bp

Back to Artabotrys

Pi 0.013

MarkerSeek score 60.00

Primer available yes

Label ndhA-ndhI

Feature Metadata

label_name: ndhA-ndhI
feature_type: igs
region: IRb
length_bp: 77
variable_sites: 1
pi: 0.013
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhA-ndhI_p1	TGACAACTTCTTCCCAACTCC	GCTCGTATTGTTTGTTGCCC	47.6	50.0	57.8	58.0	162 bp	1.0	70.6
ndhA-ndhI_p2	CTTCCCAACTCCTTTCACTGT	GCTCGTATTGTTTGTTGCCC	47.6	50.0	57.8	58.0	153 bp	1.0	70.6
ndhA-ndhI_p3	TGACAACTTCTTCCCAACTCC	CGATTCGCGTGAGATAAGGT	47.6	50.0	57.8	57.9	213 bp	1.0	69.7
ndhA-ndhI_p4	CTTCCCAACTCCTTTCACTGT	CGATTCGCGTGAGATAAGGT	47.6	50.0	57.8	57.9	204 bp	1.0	69.7
ndhA-ndhI_p5	TGACAACTTCTTCCCAACTCC	ACCAATGTACCTTGCAGCTC	47.6	50.0	57.8	58.2	178 bp	1.0	69.4