Hotspot

trnF(GAA)-ndhJ

LSC · 51910-52621 · 712 bp

Back to Artabotrys

Pi 0.00421

MarkerSeek score 61.20

Primer available yes

Label trnF(GAA)-ndhJ

Feature Metadata

label_name: trnF(GAA)-ndhJ
feature_type: igs
region: LSC
length_bp: 712
variable_sites: 3
pi: 0.00421
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnF(GAA)-ndhJ_p1	GGGATAGCTCAGCTGGTAGA	ATCCACGCCTGAAACGTATC	55.0	50.0	58.0	58.1	886-903 bp	1.0	79.7
trnF(GAA)-ndhJ_p2	CTCTAGCGGGATGATGCATC	ATCCACGCCTGAAACGTATC	55.0	50.0	58.0	58.1	916-933 bp	1.0	79.7
trnF(GAA)-ndhJ_p3	CCTCGTGTCACCAGTTCAAA	ATCCACGCCTGAAACGTATC	50.0	50.0	58.1	58.1	850-867 bp	1.0	79.4
trnF(GAA)-ndhJ_p4	TAGACCCAAGTCCTCTAGCG	ATCCACGCCTGAAACGTATC	55.0	50.0	57.9	58.1	928-945 bp	1.0	79.3
trnF(GAA)-ndhJ_p5	GGGATAGCTCAGCTGGTAGA	TGAAAGTTGGATAGGCTGGC	55.0	50.0	58.0	57.9	858-875 bp	1.0	79.2