Hotspot

trnT(TGT)-trnL(TAA)

LSC · 49709-50895 · 1187 bp

Back to Artabotrys

Pi 0.0152

MarkerSeek score 53.70

Primer available yes

Label trnT(TGT)-trnL(TAA)

Feature Metadata

label_name: trnT(TGT)-trnL(TAA)
feature_type: igs
region: LSC
length_bp: 1187
variable_sites: 18
pi: 0.0152
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 53.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT(TGT)-trnL(TAA)_p1	TTCCGGGTCTAGGCCTTTTA	TACGTACGTACACGAGGTCA	50.0	50.0	58.0	57.9	2037-2046 bp	1.0	80.0
trnT(TGT)-trnL(TAA)_p2	TTCCGGGTCTAGGCCTTTTA	AACACAGGATTTGGCTCAGG	50.0	50.0	58.0	58.1	1885-1894 bp	1.0	80.0
trnT(TGT)-trnL(TAA)_p3	GGAAGTTCCCGCGACATAAT	TACGTACGTACACGAGGTCA	50.0	50.0	58.1	57.9	1672-1686 bp	1.0	79.9
trnT(TGT)-trnL(TAA)_p4	TTCCGGGTCTAGGCCTTTTA	CGTACGTACACGAGGTCATC	50.0	55.0	58.0	57.9	2035-2044 bp	1.0	79.9
trnT(TGT)-trnL(TAA)_p5	TTCCGGGTCTAGGCCTTTTA	CGAGGTCATCCTTTCTGTCG	50.0	55.0	58.0	58.1	2025-2034 bp	1.0	79.9