Hotspot

trnC(GCA)-petN

LSC · 29608-30659 · 1052 bp

Back to Artabotrys

Pi 0.00956

MarkerSeek score 61.90

Primer available yes

Label trnC(GCA)-petN

Feature Metadata

label_name: trnC(GCA)-petN
feature_type: igs
region: LSC
length_bp: 1052
variable_sites: 10
pi: 0.00956
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 61.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnC(GCA)-petN_p1	TTTTGTATCGTTTTGGCGGC	TACCCCTAGAGTCCACTCCT	45.0	55.0	57.9	58.0	1231-1233 bp	1.0	80.6
trnC(GCA)-petN_p2	TTTTGTATCGTTTTGGCGGC	TTAAAGCAGCCCAAGCAAGA	45.0	45.0	57.9	58.0	1170-1172 bp	1.0	80.6
trnC(GCA)-petN_p3	ATCCCTTTCTCCCCAGTTCA	TACCCCTAGAGTCCACTCCT	50.0	55.0	57.9	58.0	1181-1183 bp	1.0	80.6
trnC(GCA)-petN_p4	ATCCCTTTCTCCCCAGTTCA	TTAAAGCAGCCCAAGCAAGA	50.0	45.0	57.9	58.0	1120-1122 bp	1.0	80.6
trnC(GCA)-petN_p5	TTTTGTATCGTTTTGGCGGC	GTACCCCTAGAGTCCACTCC	45.0	60.0	57.9	57.9	1232-1234 bp	1.0	80.0