Hotspot

rps16

LSC · 4549-5664 · 1116 bp

Back to Arrhenatherum

Pi 0

MarkerSeek score 40.00

Primer available yes

Label rps16

Feature Metadata

label_name: rps16
feature_type: gene
region: LSC
length_bp: 1116
variable_sites: 0
pi: 0
unique_haplotype_count: 1
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 40.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16_p1	ATGTTGGATTGGCACGACAT	ACCACTTTGCAATGAAAAGAGG	45.0	40.9	58.2	57.7	1259 bp	1.0	60.1
rps16_p2	GCTTATGTTGGATTGGCACG	ACCACTTTGCAATGAAAAGAGG	50.0	40.9	57.8	57.7	1263 bp	1.0	59.8
rps16_p3	ATGTTGGATTGGCACGACAT	CCACTTTGCAATGAAAAGAGGA	45.0	40.9	58.2	57.4	1258 bp	1.0	58.0
rps16_p4	GCTTATGTTGGATTGGCACG	CCACTTTGCAATGAAAAGAGGA	50.0	40.9	57.8	57.4	1262 bp	1.0	57.7
rps16_p5	AGGGGCTTATGTTGGATTGG	ACCACTTTGCAATGAAAAGAGG	50.0	40.9	57.5	57.7	1267 bp	1.0	57.7