Hotspot

rpoB

LSC · 19279-22509 · 3231 bp

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Pi 0.00031

MarkerSeek score 55.40

Primer available yes

Label rpoB

Feature Metadata

label_name: rpoB
feature_type: gene
region: LSC
length_bp: 3231
variable_sites: 1
pi: 0.00031
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 55.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB_p1	GGGGGAGAACTTGCTTTAGG	TTCTGTCCAAATTGCGGAGT	55.0	45.0	57.9	57.7	235 bp	1.0	77.1
rpoB_p2	GGGGGAGAACTTGCTTTAGG	TTCTACCCAGGATCCCAACC	55.0	55.0	57.9	58.4	267 bp	1.0	76.0
rpoB_p3	GGGGGAGAACTTGCTTTAGG	TCACCAGTTTCTACCCAGGA	55.0	50.0	57.9	57.6	275 bp	1.0	76.0
rpoB_p4	GTTGGGGGAGAACTTGCTTT	TTCTGTCCAAATTGCGGAGT	50.0	45.0	58.3	57.7	238 bp	1.0	75.8
rpoB_p5	GGGGGAGAACTTGCTTTAGG	CTACCCAGGATCCCAACCTA	55.0	55.0	57.9	57.5	265 bp	1.0	75.3