Hotspot

psbD

LSC · 8562-9623 · 1062 bp

Back to Arrhenatherum

Pi 0

MarkerSeek score 40.00

Primer available yes

Label psbD

Feature Metadata

label_name: psbD
feature_type: gene
region: LSC
length_bp: 1062
variable_sites: 0
pi: 0
unique_haplotype_count: 1
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 40.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbD_p1	GACATGAGGTGCTCGGAAAT	AGTGGTTTCTTGGTCACGAC	50.0	50.0	58.0	58.1	1151 bp	1.0	79.4
psbD_p2	GACATGAGGTGCTCGGAAAT	CAGTGGTTTCTTGGTCACGA	50.0	50.0	58.0	58.1	1152 bp	1.0	79.3
psbD_p3	GACATGAGGTGCTCGGAAAT	TCCAAGTAGCTTACCGGACA	50.0	50.0	58.0	58.1	1211 bp	1.0	79.2
psbD_p4	TTGGAAGTGACATGAGGTGC	AGTGGTTTCTTGGTCACGAC	50.0	50.0	58.1	58.1	1159 bp	1.0	78.8
psbD_p5	TTGGAAGTGACATGAGGTGC	CAGTGGTTTCTTGGTCACGA	50.0	50.0	58.1	58.1	1160 bp	1.0	78.8