Hotspot

ndhH

IRb · 101915-102121 · 207 bp

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Pi 0.0531

MarkerSeek score 81.60

Primer available yes

Label ndhH

Feature Metadata

label_name: ndhH
feature_type: gene
region: IRb
length_bp: 207
variable_sites: 11
pi: 0.0531
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 81.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhH_p1	ATCCCGTGAAATTCTCGAGC	AGTTGGTCGTATAATCGCGG	50.0	50.0	58.1	57.9	1247-1255 bp	1.0	79.2
ndhH_p2	ATCCCGTGAAATTCTCGAGC	GTTGGTCGTATAATCGCGGT	50.0	50.0	58.1	58.2	1246-1254 bp	1.0	79.0
ndhH_p3	ATCCCGTGAAATTCTCGAGC	ATGTAGGAGGAGGGCGAATT	50.0	50.0	58.1	58.2	1081-1089 bp	1.0	78.8
ndhH_p4	ATCCCGTGAAATTCTCGAGC	TCCCCTTTCCTATCCCCTTC	50.0	55.0	58.1	57.8	976-984 bp	1.0	78.8
ndhH_p5	ATCCCGTGAAATTCTCGAGC	AATGGAGCGACGGATAATGG	50.0	50.0	58.1	57.8	1529-1537 bp	1.0	78.7