Hotspot

atpA

LSC · 33945-35468 · 1524 bp

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Pi 0.000656

MarkerSeek score 59.10

Primer available yes

Label atpA

Feature Metadata

label_name: atpA
feature_type: gene
region: LSC
length_bp: 1524
variable_sites: 1
pi: 0.000656
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpA_p1	TTCCCCTAGCTTCCGAAAAA	ACTTGTTTGCATGATTCAAATGA	45.0	30.4	56.7	55.9	1654 bp	1.0	40.0
atpA_p2	TTTCCCCTAGCTTCCGAAAA	ACTTGTTTGCATGATTCAAATGA	45.0	30.4	56.7	55.9	1655 bp	1.0	40.0
atpA_p3	TTTTCCCCTAGCTTCCGAAA	ACTTGTTTGCATGATTCAAATGA	45.0	30.4	56.7	55.9	1656 bp	1.0	40.0
atpA_p4	TTTTTCCCCTAGCTTCCGAA	ACTTGTTTGCATGATTCAAATGA	45.0	30.4	56.7	55.9	1657 bp	1.0	40.0
atpA_p5	TTCCCCTAGCTTCCGAAAAA	AGAAAACTTGTTTGCATGATTCA	45.0	30.4	56.7	55.8	1659 bp	1.0	40.0