Hotspot

rps15-trnN-GUU

IRa · 115284-116841 · 1558 bp

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Pi 0
MarkerSeek score 58.90
Primer available yes
Label rps15-trnN-GUU

Feature Metadata

label_name
rps15-trnN-GUU
feature_type
igs
region
IRa
length_bp
1558
variable_sites
0
pi
0
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
58.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps15-trnN-GUU_p1 AGCAAGTCTCCGTATCTTATTGG AGGATTCGAACCTACGACCA 43.5 50.0 58.0 58.2 1735-1740 bp 1.0 54.6
rps15-trnN-GUU_p2 AGCAAGTCTCCGTATCTTATTGG GGATTCGAACCTACGACCAG 43.5 55.0 58.0 57.8 1734-1739 bp 1.0 54.2
rps15-trnN-GUU_p3 AGCAAGTCTCCGTATCTTATTGG CCCCAAGTAGGATTCGAACC 43.5 55.0 58.0 57.7 1743-1748 bp 1.0 53.2
rps15-trnN-GUU_p4 GCAAGTCTCCGTATCTTATTGGT AGGATTCGAACCTACGACCA 43.5 50.0 58.3 58.2 1734-1739 bp 1.0 52.5
rps15-trnN-GUU_p5 GCAAGTCTCCGTATCTTATTGG AGGATTCGAACCTACGACCA 45.5 50.0 56.7 58.2 1734-1739 bp 1.0 52.4